食品科学 ›› 2009, Vol. 30 ›› Issue (23): 326-330.doi: 10.7506/spkx1002-6300-200923074

• 生物工程 • 上一篇    下一篇

提高酸乳中c9,t11- 共轭亚油酸含量的研究

李素波1,靳小攀1,郑玉梅2,高红伟1,檀英霞1,郁成雨1,陈炳卿3,王璇琳1 ,*   

  1. 1.军事医学科学院野战输血研究所 2.国家人口计划生育委员会科学技术研究所,中国计划生育学杂志社 3.哈尔滨医科大学公共卫生学院
  • 收稿日期:2008-12-26 出版日期:2009-12-01 发布日期:2010-12-29
  • 通讯作者: 王璇琳 E-mail:wxl-ss@163.com

Production of c9, t11-Conjugated Linoleic Acid in Yogurt

LI Su-bo1,JIN Xiao-pan1,ZHENG Yu-mei2,GAO Hong-wei1,CHAN Ying-xia1,YU Cheng-yu1,CHEN Bing-qing3,WANG Xuan-lin1,*   

  1. 1. Beijing Institute of Transfusion Medicine, Academy of Military Medical Sciences, Beijing 100850, China;
    2. National Research Institute for Family Planning, Chinese Journal of Family Planning, Beijing 100081, China;
    3. College of Public Health, Harbin Medical University, Harbin 150086, China
  • Received:2008-12-26 Online:2009-12-01 Published:2010-12-29
  • Contact: WANG Xuan-lin E-mail:wxl-ss@163.com

摘要:

本研究利用乳酸菌发酵法,添加合适的底物,在形成酸乳的同时提高乳中c9,t11- 共轭亚油酸,c9,t11-CLA的含量,筛选菌株的最佳配比条件,研究影响CLA 含量的因素,发现奶中影响酶促反应和菌株生长的营养成分均可以影响乳酸菌发酵中CLA 的产生。应用气相色谱检测出乳酸菌培养液中CLA 的其它异构体。

关键词: c9,t11-共轭亚油酸, 乳酸菌发酵法

Abstract:

The research wsa aimed to increase the yield of conjugated-linoleic acid (CLA) in yogurt. The fermentation conditions for production of CLA were optimized and the factors including substrates and the mixing proportion of the Lactobacillus straims were investigated. The results showed that the nutriton composition significantly affected the enzumecatalyzed reaction and the lactobacillus growth as well as the CLA yield. Additionally, other CLA isomers besides c9, t11- CLA were identified by improved GC in the culture medium. The method provided novel insights in developing the milk products abundant in CLA.

Key words: c9, t11-conjugated linoleic acid (CLA), fermentation of Lactobacillus

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