食品科学 ›› 2009, Vol. 30 ›› Issue (22): 153-156.doi: 10.7506/spkx1002-6300-200922033

• 工艺技术 • 上一篇    下一篇

丝素蛋白乙酰化修饰工艺研究

程海涛,李军生,韦 飞,阎柳娟   

  1. 广西工学院生物与化学工程系
  • 收稿日期:2008-11-12 修回日期:2009-05-12 出版日期:2009-11-15 发布日期:2010-12-29
  • 通讯作者: 程海涛 E-mail:chenghaitao123456@163.com
  • 基金资助:

    广西科学研究与技术开发计划课题(桂科合0815007-1-2)

Acetylation Modification of Fibroin Protein

CHENG Hai-tao,LI Jun-sheng,WEI Fei,YAN Liu-juan   

  1. Department of Biological and Chemical Engineering, Guangxi University of Technology, Liuzhou 545006, China
  • Received:2008-11-12 Revised:2009-05-12 Online:2009-11-15 Published:2010-12-29
  • Contact: CHENG Hai-tao, E-mail:chenghaitao123456@163.com

摘要:

研究丝素蛋白乙酰化的工艺条件,主要对丝素蛋白乙酰化工艺参数——丝素蛋白浓度、乙酸酐用量、反应时间、反应温度、反应pH 值作探索,用正交试验确定最佳的工艺参数。结果表明,乙酰化的最佳工艺条件为丝素蛋白浓度2%、乙酸酐用量15%、反应时间20min、反应温度60℃、反应pH8.5,乙酰化程度达到47.8%。乙酰化的丝素蛋白可以作为优良的表面活性剂。

关键词: 丝素蛋白, 乙酰化, 修饰, 工艺

Abstract:

Fibroin protein was modified by acetylation, and some key technique parameters such as fibroin protein concentration, acetic anhydride dosage, reaction temperature, time and pH value were studied and optimized by conducting orthogonal test. Results showed that the optimum acetylation reaction was carried out at 60 ℃, pH 8.5 for 20 min with fibroin protein concentration 2% and anhydride concentration 15%. Under such conditions, the acetylation ratio was up to 47.8%.

Key words: fibroin protein, acetylation, modification

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