食品科学 ›› 2009, Vol. 30 ›› Issue (22): 314-317.doi: 10.7506/spkx1002-6300-200922074

• 分析检测 • 上一篇    下一篇

分光光度法测定落叶松中的总黄酮含量

王 宇,王 遂*   

  1. 哈尔滨师范大学化学化工学院
  • 收稿日期:2008-12-04 出版日期:2009-11-15 发布日期:2010-12-29
  • 通讯作者: 王遂 E-mail:wangsuiyzl@126.com

Determination of Total Flavonoids in Larch by Spectrophotometry

WANG Yu,*   

  1. College of Chemistry and Chemical Engineering, Harbin Normal University, Harbin 150025, China
  • Received:2008-12-04 Online:2009-11-15 Published:2010-12-29
  • Contact: WANG Sui E-mail:wangsuiyzl@126.com

摘要:

在高效液相色谱方法测定落叶松中二氢槲皮素含量的基础上,对测定植物提取液中总黄酮含量的三种分光光度法的准确性进行比较。结果为直接测量法较硝酸铝法、三氯化铝法能够更加准确地测量出落叶松提取液中总黄酮的含量,其回归方程为Y = 53.2714X + 0.01053(r = 0.9999),表明溶液质量浓度在0.004~0.024mg/ml 内呈良好线性关系,精密度RSD 为1.36%(n = 5),平均回收率为99.9%。

关键词: 分光光度法, 落叶松, 总黄酮

Abstract:

The determination accuracy of flavonoids in larch using three kinds of spectrophotometry methods was compared on the basis of determination of dihydroquercetin in larch using HPLC. Results indicated that direct determination of flavoniods in larch exhibited more accuracy than Al(NO3)3 and AlCl3 colorimetry. The linear equation was Y = 53.2714X+0.0153 (r= 0.9999). The excellent linear was shown in the range of 0.004-0.024 mg/ml. The relative standard deviation of this determination method was 1.36% and average recovery rate was 99.9%.

Key words: spectrophotometry, larch, total flavonoids

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