食品科学 ›› 2009, Vol. 30 ›› Issue (22): 286-289.doi: 10.7506/spkx1002-6300-200922067

• 分析检测 • 上一篇    下一篇

高效液相色谱法测胆固醇含量鉴别地沟油

郭 涛,杜蕾蕾,万 辉,何东平*   

  1. 武汉工业学院食品科学与工程学院
  • 收稿日期:2008-12-16 出版日期:2009-11-15 发布日期:2010-12-29
  • 通讯作者: 何东平 E-mail:guotao1-1@163.com
  • 基金资助:

    武汉工业学院研究生创新基金项目(07cx004)

Identification of Hogwash Oil-adulterated Rapeseed Oil through HPLC Detection of Cholesterol Content

GUO Tao,DU Lei-lei,WAN Hui,HE Dong-ping*   

  1. College of Food Science and Engineering,Wuhan Polytechnic University, Wuhan 430023, China
  • Received:2008-12-16 Online:2009-11-15 Published:2010-12-29
  • Contact: HE Dong-ping E-mail:guotao1-1@163.com

摘要:

利用高效液相色谱法测定地沟油中胆固醇含量,继而定性分析食用油中是否掺地沟油。实验结果表明,当待检菜籽油样品中胆固醇的含量大于0.05mg/g 时,可以很明显地观察到胆固醇色谱峰,且菜籽油中胆固醇含量随地沟油的增加呈线性增加,而合格的食用植物油未检测出胆固醇。可利用此特征作为判断植物油中是否掺混了地沟油的依据。

关键词: 菜籽油, 地沟油, 胆固醇, 色谱

Abstract:

Cholesterol content was determined with HPLC method for identification of hogwash oil-adulterated rapeseed oil. A good linear correlation between the adulteration amount of hogwash oil and the cholesterol content, and cholesterol was not detected in qualified rapeseed oil. Thus cholesterol content could be used as an indicator for identification of oil adulterated with hogwash oil with the lowest detection limit of 0.05 mg/g.

Key words: rape seed oil, hogwash oil, cholesterol, chromatography

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