食品科学 ›› 2009, Vol. 30 ›› Issue (20): 169-172.doi: 10.7506/spkx1002-6300-200920033

• 工艺技术 • 上一篇    下一篇

苏丹红Ⅰ结构类似物合成及其人工抗原的制备研究

孟 玮,刘仁荣*,裘雪梅,涂 顺,谢少霞,吴前辉   

  1. 江西科技师范学院生命科学学院
  • 收稿日期:2008-11-28 修回日期:2009-07-06 出版日期:2009-10-15 发布日期:2010-12-29
  • 通讯作者: 刘仁荣 E-mail:lilirenrong@hotmail.com
  • 基金资助:

    江西科技师范学院博士科研启动基金项目;江西科技师范学院大学生科研创业项目(08200706)

Synthesis of Sudan I Analog and Preparation of its Artificial Antigen

MENG Wei,LIU Ren-rong*,QIU Xue-mei,TU Shun,XIE Shao-xia,WU Qian-hui   

  1. College of Life Science, Jiangxi Science and Technology Normal University, Nanchang 330013, China
  • Received:2008-11-28 Revised:2009-07-06 Online:2009-10-15 Published:2010-12-29
  • Contact: LIU Ren-rong*, E-mail:lilirenrong@hotmail.com

摘要:

采用重氮法合成苏丹红Ⅰ的结构类似物,通过活性酯法将其偶联到载体蛋白上制备人工抗原,竞争ELISA方法测定人工抗原的免疫原性。紫外可见光谱、红外光谱和核磁共振鉴定成苏丹红Ⅰ类似物合成成功并顺利偶联到载体蛋白上,免疫原性鉴定证明该人工抗原可刺激BALB/c小鼠产生苏丹红Ⅰ的抗体,表明苏丹红Ⅰ人工抗原制备成功。

关键词: 苏丹红Ⅰ, 人工抗原, 免疫检测

Abstract:

A Sudan I analog was synthesized by diazotization and its artificial antigen was prepared by conjugating a carrier protein to the Sudan I analog using an active ester method. Immunogenity of the artificial antigen was determined through a competitive enzyme-linked immunosorbent assay (ELISA). UV, FT-IR and NMR spectra exhibited a successful synthesis of Sudan I analog and an effective coupling to the carrier protein. Antibodies against Sudan I were generated in serum of immunized BALB/c mice with prepared artificial antigen. All of these investigations revealed a successful preparation of Sudan I artificial antigen.

Key words: Sudan I, artificial antigen, ELISA

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