食品科学 ›› 2009, Vol. 30 ›› Issue (19): 177-180.doi: 10.7506/spkx1002-6630-200919039

• 生物工程 • 上一篇    下一篇

鼠李糖乳杆菌菌株LR12和LR76抗氧化性研究

王玉华1,王丽梅,冯印2,高晶2,胡耀辉2   

  1. 1. 吉林农业大学食品科学与工程学院
    2. 常熟理工学院发酵工程技术研究中心
  • 收稿日期:2008-08-04 修回日期:2009-07-22 出版日期:2009-10-01 发布日期:2010-12-29
  • 通讯作者: 胡耀辉2 E-mail:huyaohui@vip.163.com
  • 基金资助:

    吉林省科技引导计划应用基础研究项目;苏州市科技发展计划(应用基础研究)项目(YJG0903)

Antioxidant Activity of Lacbacillus rhamnosus LR12 and LR76

WANG Yu-hua1 WANG Li-mei2 FENG Yin1 GAO Jing1 HU Yao-hui1,*   

  1. 1. College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China
    2. Center of Fermentation Engineering Technology Research, Changshu Institute of Technology, Changshu 215500, China
  • Received:2008-08-04 Revised:2009-07-22 Online:2009-10-01 Published:2010-12-29
  • Contact: HU Yao-hui1,* E-mail:huyaohui@vip.163.com

摘要:

本研究目的是以耐受过氧化氢试验筛选抗氧化性强鼠李糖乳杆菌菌株,并对筛选菌株进行过氧化氢耐受能力、清除羟自由基能力和超氧化阴离子自由基能力进行表征。结果获得两株抗过氧化氢能力强的鼠李糖乳杆菌菌株LR12和LR76,两菌株在1.0mmol/L的过氧化氢溶液中2小时内具有良好的耐受性;LR12和LR76培养物对清除羟自由基清除率分别为48%和55%,对超氧化阴离子自由基清除率分别为57%和68%,LR76菌株清除羟自由基和超氧化阴离子自由基能力强于阳性对照鼠李糖乳杆菌LGG,LR76菌株略低于LGG。说明两菌株具有高效清除羟自由基和超氧化阴离子自由基能力;初步研究表明两菌株起抗氧化作用的活性成分可能与活细胞和细胞内活性成分有密切的关系。

关键词: 鼠李糖乳杆菌, 抗氧化, 羟基自由基, 超氧化阴离子自由基

Abstract:

In order to screen strains with strong antioxidant activity from Lacbacillus rhamnosus, H2O2 tolerance capability was applied to evaluate antioxidant activity. Two desired strains (LR12 and LR76) were successfully identified. H2O2 tolerance capability, hydroxyl free radical and superoxide anion elimination ability were determined for the characterization of both strains. Results indicated that LR12 and LR76 had excellent tolerance in 1.0 mmol/L H2O2 solution up to 2 h. Scavenging efficiencies of LR12 and LR76 were 48% and 55% for hydroxyl free radicals, 57% and 68% for superoxide anions respectively. LR76 had higher efficiency on hydroxyl free radical and superoxide anion scavenging than L. rhamnosus LGG, while LR12 was a little lower than LGG. Therefore, antioxidant activity of LR12 and LR76 should be related to active cells or active components in cells.

Key words: Lactobacillus rhamnosus , antioxidant activity , H2O2 tolerance capability, hydroxyl free radical , superoxide anion radicals

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