食品科学 ›› 2009, Vol. 30 ›› Issue (19): 232-235.doi: 10.7506/spkx1002-6630-200919053

• 生物工程 • 上一篇    下一篇

两株产抑菌物质乳酸菌的鉴定及系统发育分析

贺银凤,靳烨 * ,田建军,吴敬,张开屏   

  1. 内蒙古农业大学食品科学与工程学院
  • 收稿日期:2008-10-31 修回日期:2009-07-31 出版日期:2009-10-01 发布日期:2010-12-29
  • 通讯作者: 靳烨 E-mail:heyinf@yahoo.com.cn
  • 基金资助:

    国家自然科学基金资助项目(29966003)

Identification and Phylogenetic Analysis of Two Stains of Lactic Acid Bacteria with Antibacterial Functions

HE Yin-feng,JIN Ye*,TIAN Jian-jun,WU Jing,ZHANG Kai-ping   

  1. College of Food Science and Engineering, Inner Mongolia Agricultural University, Huhhot 010018, China
  • Received:2008-10-31 Revised:2009-07-31 Online:2009-10-01 Published:2010-12-29
  • Contact: JIN Ye E-mail:heyinf@yahoo.com.cn

摘要:

对分离自酸马奶酒中的两株产抑菌物质的乳酸菌BY-3-2、SQ-3-2进行分离鉴定及系统发育分析,以更准确地判定其属种。采用菌株生理、生化特性鉴定及其16S rDNA 序列分析。结果表明,菌株BY-3-2、SQ-3-2的生理、生化特性符合肠球菌属特征,其中BY-3-2菌株符合E.faecium的特征;SQ-3-2菌株符合E.durans的特征。通过16S rDNA 序列进行系统发育分析,结果与生理生化鉴定相一致。

关键词: 乳酸菌, 分离鉴定, 16S rDNA 序列分析

Abstract:

In order to identify genus and species of two stains of lactic acid bacteria with antibacterial functions, BY-3-2 and SQ-3-2 were separated from Koumiss. Their physiological and biochemical characteristics and 16S rDNA sequence were analyzed. Results indicated that physiological and biochemical characteristics of BY-3-2 and SQ-3-2 were the same as those of Enterococcus. Moreover, strain BY-3-2 had the characteristics of E. faecium, and SQ-3-2 had the characteristics of E. durans, which was also supported by 16S rDNA sequence analysis.

Key words: lactic acid bacteria , isolation and identification , 16S rDNA sequence analysis

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