食品科学 ›› 2009, Vol. 30 ›› Issue (17): 212-216.doi: 10.7506/spkx1002-6630-200917049

• 生物工程 • 上一篇    下一篇

永丰辣酱自然发酵过程中主要优势菌及化学成分的动态变化分析

苏东林1,2,张忠刚3,陈 亮4,谭 欢1,2,周向荣5   

  1. 1.湖南省农产品加工研究所 2.湖南省食品测试分析中心 3.寿光市质量技术监督局 4.湖南省原子能农业应用研究所 5.湖南省标准化研究院
  • 收稿日期:2008-09-19 修回日期:2009-06-17 出版日期:2009-09-01 发布日期:2014-04-14
  • 通讯作者: 苏东林 E-mail:sdl791228@163.com

Dominant Microbes and Dynamic Analysis of Chemical Components during Spontaneous Fermentation of Yongfeng Hot Pepper Sauce

SU Dong-lin1,2,ZHANG Zhong-gang3,CHEN Liang4,TAN Huan1,2,ZHOU Xiang-rong5   

  1. 1. Hunan Agricultural Product Processing Institute, Changsha 410125, China;2. Hunan Food Test and Analysis Center, Changsha
    410125, China;3. Shouguang Bureau of Quality and Technical Supervision, Shouguang 262700, China;
    4. Hunan Institute of Atomic Energy Application in Agriculture, Changsha 410125, China;5. Hunan Standardization Institute,
    Changsha 410007, China)
  • Received:2008-09-19 Revised:2009-06-17 Online:2009-09-01 Published:2014-04-14
  • Contact: SU Dong-lin1 E-mail:sdl791228@163.com

摘要:

实验对传统永丰辣酱的基础原料麦酱在自然发酵不同时期的微生物进行分离、培养、计数、归类和鉴定,以及对氨基态氮和蛋白酶活力、总酯与糖化酶活力、酸度与pH 值进行测定和分析。结果表明:传统产品生产过程中的各类微生物在不同的生产阶段起着不同的作用;米曲霉、酱油曲霉、黑曲霉的大量繁殖主要存在于制酱的第一阶段即制曲阶段,其作用主要是产生蛋白酶和糖化酶等多种酶类,为分解原料中的蛋白质、淀粉等大分子物质创造条件;鲁氏酵母和盐水四联球菌的繁殖则积累大量酒精、乳酸等中间物质,为酱的成熟准备条件;第三阶段是酱的成熟阶段,也是酱香形成的主要阶段。

关键词: 永丰辣酱, 自然发酵, 优势菌, 化学成分, 分析

Abstract:

Dominant microbes were separated from Yongfeng hot pepper sauce fermented system and identified and the biochemical parameters of fermented hot pepper sauce such as amino nitrogen content, protease activity, total ester content, glucoamylase activity, acidity and pH were analyzed at different fermentation stages. Microbes played different roles at different fermentation stages. The propagation of Aspergillus oryzae, Aspergillus niger and Aspergillus sojae was observed during the initial stage of fermentation, which led to the synthesis of proteases and glucoamylase. However, the stains of Saccharomyces rouxii and Tetrcoccus soyae may contribute to large-scale accumulation of many intermediate products like alcohol and lactic acid. The last mature stage of product was also the formation stage of the unique aroma of Yongfeng fermented hot pepper sauce.

Key words: Yongfeng hot pepper sauce, fermentation, dominant microbe, chemical components, analysis

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