食品科学 ›› 2009, Vol. 30 ›› Issue (17): 195-199.doi: 10.7506/spkx1002-6630-200917045

• 生物工程 • 上一篇    下一篇

枯草芽孢杆菌水解玉米蛋白粉培养基的优化

吴泽柱,曹龙奎*   

  1. 黑龙江八一农垦大学食品学院,黑龙江省农产品加工工程技术研究中心
  • 收稿日期:2008-10-17 修回日期:2009-07-09 出版日期:2009-09-01 发布日期:2014-04-14
  • 通讯作者: 曹龙奎* E-mail:longkuicao@yahoo.com.cn
  • 基金资助:

    黑龙江省农垦总局“十一五”课题(HNKXIV-03-05)

Optimization of Fermentation Medium of Bacillus subtilis for Hydrolysis of Corn Gluten Meal

WU Ze-zhu,CAO Long-kui   

  1. College of Food Science, Heilongjiang August First Land Reclamation University, Agri-Food Processing Development Centre of
    Heilongjiang, Daqing 163319, China
  • Received:2008-10-17 Revised:2009-07-09 Online:2009-09-01 Published:2014-04-14
  • Contact: CAO Long-kui E-mail:longkuicao@yahoo.com.cn

摘要:

采用单因素试验和五元二次旋转正交试验设计对枯草芽孢杆菌k-1 水解玉米蛋白粉的培养基进行优化。以玉米蛋白粉水解度为指标,对培养基中碳源、氮源、无机盐、表面活性剂及其添加量对玉米蛋白粉水解度的影响进行研究;在此基础上,采用五元二次旋转正交试验设计对影响玉米蛋白粉发酵的关键影响因子进行优化,确定最佳的发酵培养基组成为:葡萄糖1.9%,蛋白胨1.9%,十二烷基磺酸钠0.12%,MgSO4 0.18%,MnSO4 0.32%,在此条件下玉米蛋白粉发酵的水解度由未优化前的9.64% 上升到11.84%,提高了23%。

关键词: 枯草芽孢杆菌, 培养基, 水解, 玉米蛋白粉

Abstract:

The single-factor method combined with a quadric regression orthogonal rotation design involving 32 experiments of five variables at five levels were applied to optimize medium compositions (i.e., carbon source, nitrogen source, inorganic salt, and surface-acting agent) of Bacillus subtilis k-1 for maximizing the degree of hydrolysis (DH) of corn gluten meal. The optimum medium compositions were determined as follows: glucose 1.9%, peptone 1.9%, sodium dodecyl sulfonate 0.12%, MgSO4 0.18% and MnSO4 0.32%. The optimized medium led to 11.84% DH, higher than 9.64% before optimization by 23%.

Key words: Bacillus subtilis, medium, hydrolysis, corn gluten meal

中图分类号: