白术多糖对局灶性脑缺血再灌注老年大鼠脑水肿的影响

doi:10.7506/spkx1002-6630-200917070

食品科学 ›› 2009, Vol. 30 ›› Issue (17): 302-304.doi: 10.7506/spkx1002-6630-200917070

白术多糖对局灶性脑缺血再灌注老年大鼠脑水肿的影响

王光伟¹,丰昀²,刘永乐³,李加兴⁴,邱细敏^1,谭敏¹

1.湖南师范大学医学院 2.长沙市中心医院 3.长沙理工大学生物与食品工程学院 4.吉首大学食品科学研究所

收稿日期:2009-01-15 修回日期:2009-06-26 出版日期:2009-09-01 发布日期:2014-04-14
通讯作者: 王光伟 E-mail:wanggwwmq323@163.com
基金资助:
湖南省自然科学基金项目(08JJ3024)；长沙市科技局科技计划资助项目(K069062-12)

Effect of Polysaccharide of Atractylodes macrocephala Koidz on Brain Edema after Focal Cerebral Ischemia Reperfusion in Aged Rats

WANG Guang-wei1，FENG Yun2，LIU Yong-le3，LI Jia-xing4，QIU Xi-min1，TAN Min1

1. College of Medicine, Hunan Normal University, Changsha 410006, China；2.Changsha Central Hospital, Changsha 410004,
China；3.College of Biological and Food Engineering, Changsha University of Science and Technology, Changsha 410015, China；
4.Institute of Food Science, Jishou University, Jishou 416000, China

Received:2009-01-15 Revised:2009-06-26 Online:2009-09-01 Published:2014-04-14
Contact: WANG Guang-wei1， E-mail:wanggwwmq323@163.com

摘要/Abstract

摘要：

目的：探讨局灶性脑缺血再灌注后应用白术多糖治疗对脑组织水肿的影响。方法：采用局灶性脑缺血再灌注模型，84 只老年雄性Sprague Dawley 大鼠随机分为对照组、再灌注组、蒸馏水组及白术多糖组，每组再根据不同的再灌注时间分为3 个亚组。取脑缺血区组织，分别检测其水、丙二醛(MDA)含量及超氧化物歧化酶(SOD)活性。结果：再灌注组、蒸馏水组及白术多糖组各时间点水分、MDA 含量均较对照组明显增加(P ＜ 0.01)，但白术多糖组各时间点水分、MDA 含量显著低于再灌注组及蒸馏水组相应时间点(P ＜ 0.01)。再灌注组、蒸馏水组及白术多糖组各时间点SOD活性均较对照组明显降低(P ＜ 0.01)，但白术多糖组各时间点SOD活性显著高于再灌注组及蒸馏水组相应时间点(P ＜ 0.01)。结论：白术多糖能通过改善SOD 活力，减少MDA 含量，进而减轻局灶性脑缺血再灌注后脑水肿的程度。

关键词: 白术多糖, 脑缺血, 再灌注, 脑水肿, 超氧化物歧化酶, 丙二醛

Abstract:

Objective: To study the effect of polysaccharide extracted from Atractylodes macrocephala Koidz on brain edema after focal cerebral ischemia reperfusion in aged rats. Methods: Focal cerebral ischemia was induced in anesthetized rats using a middle cerebral artery occlusion (MCAO) model. Eighty-four male Sprague Dawley rats were randomly divided into four groups: sham group, reperfusion group, distilled water group and Atractylodes macrocephala Koidz polysaccharide group. Then three subgroups of rats were obtained at 1 h of MCAO with different time intervals (24, 72 and 120 h) of reperfusion. The contents of water and malondialdehyde (MDA) and the superoxide dismutase (SOD) activity were detected in the ischemic brain tissues. Results: The contents of water and MDA in reperfusion group, distilled water group and Atractylodes macrocephala Koidz polysaccharide group both were significantly higher than those in sham group at different time intervals of reperfusion (P < 0.01) and these items in Atractylodes macrocephala Koidz polysaccharide group were significantly lower than those in reperfusion group and distilled water group (P < 0.01). The SOD activity in reperfusion group, distilled water group and Atractylodes macrocephala Koidz polysaccharide group was significantly lower than that in sham group (P < 0.01) and the SOD activity in Atractylodes macrocephala Koidz polysaccharide group was significantly higher than that in reperfusion group and distilled water group (P < 0.01). Conclusions: Atractylodes macrocephala Koidz polysaccharide is able to markedly increase SOD activity, decrease MDA and alleviate brain edema in the ischemic areas.

Key words: polysaccharide of Atractylodes macrocephala Koidz, brain ischemia, reperfusion, brain edema, superoxide dismutase, malondialdehyde

中图分类号:

TS201.23

王光伟1，丰昀2，刘永乐3，李加兴4，邱细敏1，谭敏1. 白术多糖对局灶性脑缺血再灌注老年大鼠脑水肿的影响[J]. 食品科学, 2009, 30(17): 302-304.

WANG Guang-wei1，FENG Yun2，LIU Yong-le3，LI Jia-xing4，QIU Xi-min1，TAN Min1. Effect of Polysaccharide of Atractylodes macrocephala Koidz on Brain Edema after Focal Cerebral Ischemia Reperfusion in Aged Rats[J]. FOOD SCIENCE, 2009, 30(17): 302-304.

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白术多糖对局灶性脑缺血再灌注老年大鼠脑水肿的影响

Effect of Polysaccharide of Atractylodes macrocephala Koidz on Brain Edema after Focal Cerebral Ischemia Reperfusion in Aged Rats

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