谷氨酰胺活性肽的理化性质及抗氧化抗疲劳效果的研究

doi:10.7506/spkx1002-6630-200917072

食品科学 ›› 2009, Vol. 30 ›› Issue (17): 308-311.doi: 10.7506/spkx1002-6630-200917072

谷氨酰胺活性肽的理化性质及抗氧化抗疲劳效果的研究

潘丽军¹，张丽¹，王珏²，方坤¹，钟昔阳¹

1.合肥工业大学生物与食品工程学院 2.安徽省轻工科学技术研究所

收稿日期:2008-11-10 修回日期:2009-06-17 出版日期:2009-09-01 发布日期:2014-04-14
通讯作者: 潘丽军 E-mail:panlijun1955@163.com
基金资助:
安徽省科技攻关重大项目(07010301018)

Physical and Chemical Properties of Glutamine-bioactive Peptides and Their Antioxidation and Anti-Fatigue Effects

PAN Li-jun1，ZHANG Li1，WANG Jue2，FANG Kun1，ZHONG Xi-yang1

1. School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China；
2. Anhui Institute of Light Industry Science Technology, Hefei 230031, China

Received:2008-11-10 Revised:2009-06-17 Online:2009-09-01 Published:2014-04-14
Contact: PAN Li-jun1， E-mail:panlijun1955@163.com

摘要/Abstract

摘要：

采用双酶分步水解法制备谷氨酰胺活性肽(glutamine-bioactive peptides，GBP)，研究其溶解度、渗透压、NaCl 含量、相对分子质量四个理化指标；并以小鼠为对象，进行GBP 的抗氧化和抗疲劳效果研究。实验设立对照组和实验组(低剂量组、中剂量组和高剂量组：分别给予200、500、800mg/kg·d 的GBP)，其中，实验组分别给予高、中、低剂量的GBP，对照组给予相同体积的生理盐水。实验小鼠分为两批，第一批连续灌胃20d，测定小鼠肝组织和肾组织中超氧化物歧化酶(SOD)活力和脂质过氧化物丙二醛(MDA)含量，第二批连续灌胃21d 后，进行小鼠游泳实验和常压耐缺氧实验，测定游泳时间和存活时间。结果显示：与对照组相比，给予高剂量GBP能显著提高小鼠肝肾组织中的SOD 活性，减少MDA 的产生；并能延长小鼠游泳时间和耐缺氧存活时间，提高抗疲劳能力。

关键词: 谷氨酰胺活性肽, 溶解度, 渗透压, 抗氧化, 抗疲劳

Abstract:

The physical and chemical properties of glutamine-bioactive peptides (GBP) which were prepared by hydrolyzing wheat protein by bienzyme method, including solubility, osmotic pressure, content of NaCl and relative molecular mass, were investigated and their antioxidation and anti-fatigue effects of glutamine-bioactive peptides on mice were evaluated. SPF Kunming mice were divided into four groups: three experimental groups (low-, medium- and high-dose groups) administered with different doses of GBP by gavage and control group administered with the same volume of normal saline instead of GBP. Gavage administration was performed in two batches: in the first batch, after 20 days of continued administration, the activity of SOD and the content of MDA in liver and kidney were measured; in the second batch, after 21 days of continued administration, the swimming time and the survival time of mice were measured by swimming test and hypoxia tolerance test, respectively. In comparison with the control group，high-dose GBP could obviously increase the activity of SOD in liver and kidney, reduce the content of MDA, prolong the swimming time and the hypoxia survival time of mice, and enhance the anti-fatigue ability.

Key words: glutamine-bioactive peptides, solubility, osmotic pressure, antioxidation, anti-fatigue

中图分类号:

TS201.24

潘丽军1，张丽1，王珏2，方坤1，钟昔阳1. 谷氨酰胺活性肽的理化性质及抗氧化抗疲劳效果的研究[J]. 食品科学, 2009, 30(17): 308-311.

PAN Li-jun1，ZHANG Li1，WANG Jue2，FANG Kun1，ZHONG Xi-yang1. Physical and Chemical Properties of Glutamine-bioactive Peptides and Their Antioxidation and Anti-Fatigue Effects[J]. FOOD SCIENCE, 2009, 30(17): 308-311.

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谷氨酰胺活性肽的理化性质及抗氧化抗疲劳效果的研究

Physical and Chemical Properties of Glutamine-bioactive Peptides and Their Antioxidation and Anti-Fatigue Effects

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