食品科学 ›› 2009, Vol. 30 ›› Issue (13): 218-222.doi: 10.7506/spkx1002-6630-200913050

• 生物工程 • 上一篇    下一篇

纤维素酶水解纤维低聚糖的研究

彭 红1,2,3,林 鹿2,刘玉环1,3,阮榕生1,3   

  1. 1.南昌大学 食品科学与技术国家重点实验室 2.华南理工大学 制浆造纸工程国家重点实验室 3.南昌大学 生物质转化教育部工程技术研究中心
  • 收稿日期:2008-10-07 修回日期:2009-01-11 出版日期:2009-07-01 发布日期:2010-12-29
  • 通讯作者: 彭红 E-mail:penghong@ncu.edu.cn; penghong.scut@yahoo.com.cn

Cellulase-catalyzed Hydrolysis of Cellooligosaccharides

PENG Hong1,2,3,LIN Lu2,LIU Yu-huan1,3,RUAN Rong-sheng1,3   

  1. 1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China;
    2. State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510640, China;
    3. Engineering Research Center for Biomass Conversion, Ministry of Education, Nanchang University, Nanchang 330047, China
  • Received:2008-10-07 Revised:2009-01-11 Online:2009-07-01 Published:2010-12-29
  • Contact: PENG Hong1,2,3, E-mail:penghong@ncu.edu.cn; penghong.scut@yahoo.com.cn

摘要:

对纤维素酶催化水解纤维三糖、纤维四糖和纤维五糖的规律进行研究。结果表明:相对水解率随低聚糖聚合度增大而降低;中间产物低聚糖和底物低聚糖竞争性与酶作用,产物中低聚合度低聚糖优先被酶催化水解;过大的纤维素酶用量并不能显著增大水解率;相对水解率均随初始低聚糖浓度增大而减小;pH5.0 时,纤维素酶最适温度为55℃。

关键词: 纤维低聚糖, 纤维素酶, 水解, 水解率, 聚合度

Abstract:

The cellulase from Aspergillus niger was used to hydrolyze cellotriose, cellotetraose and cellopentaose. The hydrolysis characteristics of the three cellooligosaccharides with different degrees of polymerization (DPs) were compared to understand the effect of DP on cellulose-catalyzed of cellooligosaccharides and explain the hydrolysis law. The results showed that the relative degree of hydrolysis (DH) decreased with the increase of DP. The oligosaccharides in intermediate products and in substrate showed competitive reaction with the enzyme and the oligosaccharides with lower DP displayed priority in the hydrolysis. Excessive usage of enzyme was unable to markedly enhance the hydrolysis. The elevated initial substrate concentration led to the decrease of relative DH. The optimum working temperatures was 55 ℃ for Aspergillus niger cellulase at pH 5.0.

Key words: cellooligosaccharides, cellulase, hydrolysis, degree of hydrolysis, degree of polymerization

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