食品科学 ›› 2009, Vol. 30 ›› Issue (13): 93-96.doi: 10.7506/spkx1002-6630-200913021

• 基础研究 • 上一篇    下一篇

酸降解水溶性银耳多糖及抗氧化作用研究

吴 琼1,代永刚2,高长城1,宁正祥3   

  1. 1.长春大学农产品加工重点实验室 2.吉林省农业科学院农产品加工研究中心 3.华南理工大学轻工与食品学院
  • 收稿日期:2008-10-09 修回日期:2009-01-16 出版日期:2009-07-01 发布日期:2010-12-29
  • 通讯作者: 吴琼 E-mail:rabbit7815@163.com

Antioxidations of Acid-degrade Water-soluble Polysaccharides from Tremella fuciformis

WU Qiong1,DAI Yong-gang2,GAO Chang-cheng1,NING Zheng-xiang3   

  1. 1. Key Laboratory of Agricultural Products Processing, Changchun University, Changchun 130022, China;
    2. Research Center of Agricultural Products Processing, Jilin Academy of Agricultural Sciences, Changchun 130033, China;
    3. College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China
  • Received:2008-10-09 Revised:2009-01-16 Online:2009-07-01 Published:2010-12-29
  • Contact: WU Qiong E-mail:rabbit7815@163.com

摘要:

采用盐酸降解法对水溶性银耳多糖进行降解,得到低分子水溶性银耳多糖。通过对DPPH 自由基清除作用,表征低分子水溶性银耳多糖抗氧化活性的变化。当溶液黏度为16.5~9.8mPa·s 时,低分子银耳多糖的平均相对分子质量在2~10kD 范围内,抗氧化活性高于水溶性银耳多糖。而当平均相对分子质量> 10kD 或平均相对分子质量< 2kD 时,抗氧化活性较低。

关键词: 银耳多糖, 降解, 抗氧化

Abstract:

The water-soluble polysaccharides extracted from Tremella fuciformis were degraded into low-molecular-weight polysaccharides using hydrochloric acid under different conditions. Then the antioxidant effects of the low-molecular-weight Tremella fuciformis polysaccharides (LTPs) obtained were investigated via the determination of DPPH radical scavenging activity in vitro. The results indicated that after the degradation in 0.1 mol/L hydrochloric acid solution at 50 ℃ for 0.5-3 h, the viscosity of the initial polysaccharides solution declined gradually from 17.8 mPa · s to 16.5-9.8 mPa · s, and the average molecular weights (MWs) of the LTPs were within the range of 2-10 kD and their antioxidant activity was higher than that of the undegraded water-soluble Tremella fuciformis polysaccharides . However, the LTPs with the average MW of higher than 10 kD or less than 2 kD, had weak antioxidant activity.

Key words: Tremella polysaccharide, degradation, antioxidation

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