食品科学 ›› 2009, Vol. 30 ›› Issue (13): 89-92.doi: 10.7506/spkx1002-6630-200913020

• 基础研究 • 上一篇    下一篇

海蜇伞部胶原蛋白的提取及理化性质的研究

庄永亮,赵 雪,张朝辉,闫鸣艳,李八方*   

  1. 中国海洋大学食品科学与工程学院
  • 收稿日期:2008-10-17 修回日期:2009-01-12 出版日期:2009-07-01 发布日期:2010-12-29
  • 通讯作者: 李八方 E-mail:qdli1999@yahoo.com.cn

Isolation and Physico-chemical Properties of Pepsin-soluble Collagen from Jellyfish Umbrella

ZHUANG Yong-liang,ZHAO Xue,ZHANG Zhao-hui,YAN Ming-yan,LI Ba-fang*   

  1. College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China
  • Received:2008-10-17 Revised:2009-01-12 Online:2009-07-01 Published:2010-12-29
  • Contact: LI Ba-fang E-mail:qdli1999@yahoo.com.cn

摘要:

研究海蜇(Rhopilema esculentum)伞部胃蛋白酶促溶性胶原蛋白的提取及其部分理化性质。氨基酸组成及SDSPAGE显示,海蜇伞部胶原蛋白(PSC)为Ⅰ型胶原蛋白;含糖量为2.8%;热变性温度和热收缩温度分别为28.8℃和51.6℃,均低于牛皮Ⅰ型胶原;红外光谱显示PSC 保留了大量的三股螺旋结构;溶解性表明PSC 的等电点为pH6,在pH3 时有最大溶解度;酸性条件下盐浓度高于4%(m/V)时,PSC 的溶解度急剧下降。

关键词: 胶原蛋白, 海蜇, 提取, 理化性质

Abstract:

Pepsin-soluble collagen (PSC) was extracted from jellyfish (Rhopilema esculentum) umbrella and its parts of physicalchemical properties were investigated. According to amino acid composition and SDS-PAGE analysis, the collagen was confirmed to belong to the type I collagen family. The content of total sugar was 2.8%, and the thermal denaturation temperature and the thermal shrinkable temperature were 28.8 ℃ and 51.6 ℃, respectively, which were lower than those of type I collagen from bovine skin. IR spectrum showed that the PSC kept a great amount of triple helix structure. Furthermore, it had the highest solubility at pH 3 and its isoelectric point was at about 6. However, when salt concentration was over 4 % (m/V) in acidic medium, the PSC solubility sharply decreased.

Key words: collagen, jellyfish (Rhopilema esculentum), isolation, property

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