食品科学 ›› 2009, Vol. 30 ›› Issue (13): 68-72.doi: 10.7506/spkx1002-6630-200913015

• 基础研究 • 上一篇    下一篇

红檵木花色素分离纯化及色素光谱性质研究

唐克华1,2,申仕康3,方 锤2   

  1. 1.吉首大学城乡资源与规划学院 2.湖南师范大学生命科学学院 3.云南大学生命科学学院
  • 收稿日期:2008-11-14 修回日期:2009-02-04 出版日期:2009-07-01 发布日期:2010-12-29
  • 通讯作者: 唐克华 E-mail:dazhongyin@hotmail.com
  • 基金资助:

    张家界市科技局高新技术引导资金课题(04HT023)

Isolation, Purification and Spectral Properties of Pigments from Loropetalum chinense var. rubrum Flower

TANG Ke-hua1,2,SHEN Shi-kang3,FANG Chui2   

  1. 1. College of Resources and Planning Sciences, Jishou University, Zhangjiajie 427000, China;
    2. College of Life Sciences, Hunan Normal University, Changsha 410081, China;
    3. School of Life Science, Yunnan University, Kunming 650091, China
  • Received:2008-11-14 Revised:2009-02-04 Online:2009-07-01 Published:2010-12-29
  • Contact: TANG Ke-hua E-mail:dazhongyin@hotmail.com

摘要:

通过对红檵木花色素的硅胶G 薄板层析(TLC)分离研究,探讨其分离条件,纯化并制备水溶性花色素的两个主要组分,对这两个组分进行黄酮类特征颜色反应及UV- 可见光谱特征定性鉴别。初步研究表明,以1.8% 羧甲基纤维素钠(CMC)制备的硅胶G 薄板最适宜红檵木花色素的层析分离,展层剂“水:乙醇:正丁醇:乙酸乙酯”的体积比为11:8:30:36 时的分离效果最佳,共分离检识出7 种水溶性花色素成分;该花色素中含花青素或花青素甙,其两个主要颜色成分均有可能属于花青素或花青素甙,但单体Ⅰ也可能属于异黄酮,单体Ⅱ则可能属于异黄酮或二氢黄酮( 醇) 。

关键词: 红檵木, 花, 色素, 薄板层析(TLC), 分离, 光谱性质

Abstract:

The pigments from Loropetalum chinense var. rubrum flower were obtained by 20% ethanol extraction followed by microwave treatment. After the multi-stage extraction with n-butanol-petroleum ether, ethyl ether and ethyl acetate, the pigments were subjected to separation by silica-G thin-layer chromatography (TLC). We analyzed detailly the spectral properties of two main fractions obtained from the pigments. The thin-layer plate containing 1.8% sodium carboxymethyl cellulose (CMC) was found to be the most effective TLC plate for separation of the pigments. The maximum separation efficiency was achieved when the developer was composed of distilled water, ethanol, n-butanol and ethyl acetate at the ratio of 11:8:30:36. Seven water-soluble components were isolated from the crude pigments (two of them as the main contributor of the pigment color). The pigments from Loropetalum chinense var. rubrum flower contained anthocyanidin. In the case of the two main color contributors of the pigments, monomer Ⅰ may be isoflavones and monomer Ⅱ may be either isoflavones or anthocyanidin.

Key words: Loropetalum chinense var. rubrum, flower, pigment, thin-layer chromatography (TLC), separation, spectral properties

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