食品科学 ›› 2009, Vol. 30 ›› Issue (13): 205-211.doi: 10.7506/spkx1002-6630-200913048

• 生物工程 • 上一篇    下一篇

利用响应面分析法优化高山被孢霉M E - 1 生产花生四烯酸培养基成分研究

彭 超1,黄 和1, 2,*,金明杰1,肖爱华2,于文涛1,2,刘 欣1   

  1. 1.南京工业大学制药与生命科学学院 2.南京工业大学 江苏省工业生物技术创新中心
  • 收稿日期:2008-08-04 修回日期:2008-10-25 出版日期:2009-07-01 发布日期:2010-12-29
  • 通讯作者: 彭超 E-mail:pengsuper_2004@163.com
  • 基金资助:

    国家“973”重点基础研究发展计划项目(2007CB707805);国家“863”计划项目(2006AA02Z240);
    国家自然科学基金项目(20576054)

Optimization of Medium Components for Biomass and Arachidonic Acid Production by Mortierella alpina ME-1 Using Response Surface Methodology

PENG Chao1,HUANG He1,2,*,JIN Ming-jie1,XIAO Ai-hua2,YU Wen-tao1,2,LIU Xin1   

  1. 1. College of Life Science and Pharmacy, Nanjing University of Technology, Nanjing 210009, China;
    2.Jiangsu Provincial Innovation Center for Industrial Biotechnology, Nanjing Univetsity of Technology, Nanjing 211816, China
  • Received:2008-08-04 Revised:2008-10-25 Online:2009-07-01 Published:2010-12-29
  • Contact: PENG Chao1, E-mail:pengsuper_2004@163.com

摘要:

在利用高山被孢霉ME-1 生产花生四烯酸(ARA)过程中,采用响应面分析法,对摇瓶中的培养基成分进行优化。建立了两个标准的多项式模型:在长达6.5d 发酵过程中,当葡萄糖、酵母膏、KH2PO4 和NaNO3 浓度分别为90.16、12.50、3.80 和3.54g/L 时,生物量将到最大,约36.86g/L;当葡萄糖、酵母膏、KH2PO4 和NaNO3浓度分别为103.16、11.66、3.80 和3.43g/L 时,AA 产量将到最大,约9.65g/L。预测值通过实验得到了充分的证实,预测值和实验结果相关性很好。发酵罐实验结果表明,在高山被孢霉ME-1 大规模发酵生产过程中,对培养基进行优化,将同时引起生物量(发酵5d,约34.21 ± 1.01g/L)和AA 产量(发酵6d,约9.86 ± 0.45g/L)的增加。

关键词: 花生四烯酸, 高山被孢霉, 优化, 响应面法, 发酵, 油脂

Abstract:

The media for biomass and arachidonic acid (ARA) production by Mortierella alpina ME-1 were optimized in shake flask cultures using response surface methodology (RSM). Two imperial polynomial models were developed, and tae maximum biomass yield of 36.86 g/L that appeared at glucose, yeast extract (YE), KH2PO4 and NaNO3 of 90.16, 12.50, 3.80 and 3.54 g/L, respectively, while a maximum ARA yield of 9.65 g/L that appeared at glucose, YE, KH2PO4 and NaNO3 of 103.16, 11.66, 3.80 and 3.43 g/L , respectively, in 6.5-day fermentation were predicted. Verified by validation experiments showed excellent correlations between their predicted and measured values. The results of bioreactor fermentation also illustrated that the optimized culture medium enhanced both biomass (34.21 ± 1.01 g/L in 5-d fermentation) and ARA (9.86 ± 0.45 g/L in 6-d fermentation) production by M. alpine ME-1 in a large-scale fermentation process.

Key words: arachidonic acid, M. alpina, optimization, response surface methodology, fermentation, lipids

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