食品科学 ›› 2009, Vol. 30 ›› Issue (13): 201-204.doi: 10.7506/spkx1002-6630-200913047

• 生物工程 • 上一篇    下一篇

酶法提取榆耳菌丝体胞内多糖的工艺研究

刘振春,蒋中华,李 慧,段 绪   

  1. 吉林农业大学食品科学与工程学院
  • 收稿日期:2008-09-23 修回日期:2009-01-13 出版日期:2009-07-01 发布日期:2010-12-29
  • 通讯作者: 刘振春 E-mail:liuzhenchun63@163.com
  • 基金资助:

    吉林省教育厅资助项目(吉教科合字2006055)

Enzymatic Extraction of Polysaccharides from Fermentation Mycelia of Gloeostereum incarnatum

LIU Zhen-chun,JIANG Zhong-hua,LI Hui,DUAN Xu   

  1. College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China
  • Received:2008-09-23 Revised:2009-01-13 Online:2009-07-01 Published:2010-12-29
  • Contact: LIU Zhen-chun, E-mail:liuzhenchun63@163.com

摘要:

本实验主要对榆耳菌菌丝体多糖提取工艺进行研究,以提高产品的得率。通过单因素试验及正交试验,得纤维素酶提取榆耳菌丝体胞内多糖的最佳工艺条件:料水比1:60、pH5.3、温度43℃、酶解时间2.0h、加酶量2.5%,在此条件下,榆耳菌丝体胞子内多糖的提取率为4.86%。

关键词: 榆耳, 多糖, 酶解, 纤维素酶

Abstract:

Cellulase-catalyzed hydrolysis was used to extract polysaccharides from the collected mycelia of Gloeostereum incarnatum 105 after submerged fermentation. The extraction technology was optimized by orthogonal factorial design based on single factor test. The results showed that the optimal conditions for extracting polysaccharides from mycelia of Gloeostereum incarnatum 105 were as follows: solid/liquid ratio 1:60, pH 5.3, cellulose amount 2.5%, and hydrolysis temperature 43 ℃ for a hydrolysis duration of 2.0 h; under these conditions, the yield of polysaccharides reached 4.86%.

Key words: Gloeostereum insarnatum, polysaccharides, enzymatic hydrolysis, cellulase

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