双相系统中硅藻土固定分枝杆菌转化甾醇的初步研究

doi:10.7506/spkx1002-6630-200913046

食品科学 ›› 2009, Vol. 30 ›› Issue (13): 197-200.doi: 10.7506/spkx1002-6630-200913046

双相系统中硅藻土固定分枝杆菌转化甾醇的初步研究

潘丽军¹，冯丽桦¹，姜绍通¹，杨英^{1 , 2}，罗炳华¹

1. 合肥工业大学生物与食品工程学院 2. 安徽建筑工业学院环境工程系

收稿日期:2008-10-14 修回日期:2009-01-13 出版日期:2009-07-01 发布日期:2010-12-29
通讯作者: 冯丽桦 E-mail:liqingfenglihua@sina.com.cn
基金资助:
“十一五” 国家科技支撑计划项目(2006BAD05A12)；安徽省自然科学基金项目(070413265X)

Conversion of Sterols into Androst-4-ene-3,17-dione (AD) by Fermentation of Mycobacterium sp. Immobilized on Diatomite in Organic-aqueous System

PAN Li-jun1，FENG Li-hua1，JIANG Shao-tong1，YANG Ying1,2，LUO Bing-hua1

(1. College of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China；
2. Department of Environmental Engineering, Anhui University of Architecture, Hefei 230022, China)

Received:2008-10-14 Revised:2009-01-13 Online:2009-07-01 Published:2010-12-29
Contact: FENG Li-hua1， E-mail:liqingfenglihua@sina.com.cn

摘要/Abstract

摘要：

在双相发酵系统中，将分枝杆菌BD696-6 细胞固定在不同载体上，进行降解植物甾醇侧链生成雄烯二酮(AD)效果的研究。结果表明：相对丝瓜布、棉纱布及泡沫，硅藻土有更好的固定化效果。在硅藻土用量7g/L，接种量18%(V/V)，甾醇添加量3g/L 的实验条件下发酵7d，AD 得率达到81.47%，比游离细胞发酵AD 得率提高了19.52%。

关键词: 雄烯二酮, 甾醇, 固定化细胞, 硅藻土

Abstract:

The microbial fermentation in organic-aqueous system is an effective method for the conservation of sterols into AD, but some related problems such as integrity of bacterial membrane and mass transfer resistance still have not been solved. In the present study, the Mycobacterium sp. BD696-6 immobilized on different carriers was used to convert sterols into AD. Compared to the strains immobilized on vegetable sponge, cotton yarn and bubble, the diatomite-immobilized strain resulted in the highest AD yield. Furthermore the conversion conditions of sterols by the fermentation of the diatomite-immobilized strain were optimized by orthogonal test design. The results showed that the optimal conditions were as follows: addition amount of diatomite to seed culture medium 7 g/L, inoculation amount 18% (V/V), concentration of sterols in fermentation medium 3 g/L, and fermentation time 7 d; under these conditions, the AD yield was up to 81.47%, which was 19.52% higher than that by the fermentation of the free strain.

Key words: androst-4-ene-3, 17-dione, sterols, immobilized strain, diatomite

中图分类号:

TS201.3

潘丽军1，冯丽桦1，姜绍通1，杨英1,2，罗炳华1. 双相系统中硅藻土固定分枝杆菌转化甾醇的初步研究[J]. 食品科学, 2009, 30(13): 197-200.

PAN Li-jun1，FENG Li-hua1，JIANG Shao-tong1，YANG Ying1,2，LUO Bing-hua1. Conversion of Sterols into Androst-4-ene-3,17-dione (AD) by Fermentation of Mycobacterium sp. Immobilized on Diatomite in Organic-aqueous System[J]. FOOD SCIENCE, 2009, 30(13): 197-200.

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双相系统中硅藻土固定分枝杆菌转化甾醇的初步研究

Conversion of Sterols into Androst-4-ene-3,17-dione (AD) by Fermentation of Mycobacterium sp. Immobilized on Diatomite in Organic-aqueous System

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