关键词: 细脚拟青霉, 活性成分, 自由基

Abstract:

After 6 d cultivation of Paecilomyces tenuipes RCEF0441 with 20 L of SDAY medium contained in 30 L fermentor, the mycelia and the broth were separated by pumping filtration. The mycelia were dried by 50 ℃ hot air, and then crushed into powder with the particle size of 80 mesh, and the broth was freeze-dried into powder. The two powders and fruit body of C. sinensis were subjected to active component analysis, and DPPH radical scavenging activity of ethyl acetate extracts of the two powders was analyzed qualitatively and quantificationally. The results showed that the contents of total amino acids, crude protein, polysaccharide, ergosterol, mannitol and adenosine in the fermentation mycelia were higher than those in fruit body of C. sinensis, and both the fermentation mycelia and the broth contained much more crude protein and total amino acids than fruit body of C. sinensis and were rich in Zn, Fe and Ca. Both the ethyl acetate extracts of the two powders presented strong DPPH radical scavenging activity, and the relative scavenging rates at 5.0 mg/ml were 61.28 % and 81.12%, respectively.

Key words: Paecilomyces tenuipes, active component, free radical