关键词: 植物瞬时表达载体, 绿色荧光蛋白基因, 共聚焦显微镜

Abstract:

The study of genomics depends on quick and high-flux screening of functional genes, and the rapid development of plant transient expression technology makes it possible to express exogenous genes in plants to widen the investigation of functional genes. The use of green fluorescent protein gene can indicate the expression of target gene, therefore we constructed a plant transient expression vector containing two separate expression frames, one with rare restriction enzyme cleavage sites for cloning and expression of target gene and the other frame with enhanced green fluorescent protein gene. Meanwhile, the vector transiently expressed green fluorescent protein gene in tobacco leaves and detached tomato fruits by agrobacterium-mediated method, indicating the potent feasibility of this vector for transient expression.

Key words: plant transient expression vector, green fluorescent protein gene, confocal microscopy