食品科学 ›› 2009, Vol. 30 ›› Issue (13): 245-249.doi: 10.7506/spkx1002-6630-200913056

• 营养卫生 • 上一篇    下一篇

鱼头中糖脂类活性物质的提取分离及抗肿瘤活性研究

曹东旭1 , 2,杨书文2,刘娟娟2,王玥玮2,刘安军1 , 2   

  1. 1.天津市食品加工工程中心 2.天津科技大学食品工程与生物技术学院
  • 收稿日期:2008-10-22 修回日期:2009-02-11 出版日期:2009-07-01 发布日期:2010-12-29
  • 通讯作者: 曹东旭 E-mail:carol@tust.edu.cn,dongxucao@sina.com
  • 基金资助:

    天津科技大学引进人才科研启动基金项目(20060422)

Isolation and Antitumor Activities of Glycolipids from Carp Head

CAO Dong-xu1,2,YANG Shu-wen2,LIU Juan-juan2,WANG Yue-wei2,LIU An-jun1,2   

  1. 1. Tianjin Food Engineering Center, Tianjin 300457, China;
    2. College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China
  • Received:2008-10-22 Revised:2009-02-11 Online:2009-07-01 Published:2010-12-29
  • Contact: CAO Dong-xu1,2, E-mail:carol@tust.edu.cn,dongxucao@sina.com

摘要:

以鲤鱼头为原料,研究其中糖脂类活性物质的提取、分离、鉴定及抗肿瘤活性。用乙醇提取粗脂,进一步用大孔吸附树脂HP-20、薄层色谱(TLC)及红外光谱对鱼头粗糖脂进行分离和初步鉴定。红外光谱分析结果初步表明,其为含有脂肪酸侧链的糖环类分子,符合糖脂的一般结构特征;细胞毒性检测(MTT 法)糖脂的抗肿瘤活性结果表明:10~400μg/ml 的鱼头糖脂作用24、48、72h 后,能显著抑制人乳腺癌细胞MCF-7 和人肝癌细胞Bel-7402 的生长。苏木精- 伊红(HE)染色结果表明,鱼头糖脂作用72h,MCF-7 和Bel-7402 细胞均产生明显的凋亡形态学变化。细胞周期分析结果表明,鱼头糖脂作用后,MCF-7 和Bel-7402 细胞被阻遏于细胞周期的S 期,且这种S 期积累具有明显的时间依赖性。

关键词: 鲤鱼头, 糖脂, 抗肿瘤活性

Abstract:

Crude glycolipids extracted from ground fresh carp head with 90% ethanol were subjected to purification by HP- 20 column chromatography and identification by thin layer chromatography (TLC) and infrared (IR) spectrum. And the antitumor activity of the glycolipids was determined by MTT assay for measuring cytotoxicity. The IR spectral analysis showed that the molecules of the analyte contaied the carbohydrate rings with fatty acyl side chain as corresponding acid, which is a general structural feature of glycolipids. Incubation of glycolipids with human breast cancer MCF-7 cells and liver cancer Bel-7402 cells for 24, 48 and 72 h significantly inhibited the growth of caner cells in vitro within the range of 10-400 μg/ml. The HE staining indicated that morphological alterations as indicative of apoptosis were observed in both MCF-7 cells and liver Bel-7402 when incubated with glycolipid extract for 72 h. Theur results suggested that, in breast and liver cancer cells, glycoplipid from carp head delays cell cycle progression by promoting accumulation of cells in S phase without affecting the rate of apoptosis and this S-phase accumulation acts in a significant time-dependent manner.

Key words: carp head, glycolipid, antitumor activity

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