食品科学 ›› 2009, Vol. 30 ›› Issue (13): 262-267.doi: 10.7506/spkx1002-6630-200913061

• 专题论述 • 上一篇    下一篇

乳酸菌有氧呼吸代谢研究进展

白凤翎1,2,张柏林1,*,蒋湘宁1   

  1. 1.北京林业大学生物科学与技术学院 2.渤海大学生物与食品科学学院
  • 收稿日期:2008-11-18 修回日期:2009-01-31 出版日期:2009-07-01 发布日期:2010-12-29
  • 通讯作者: 张柏林 E-mail:zhangbolin888@126.com
  • 基金资助:

    国家“863”计划项目(2006AA10Z344;2008AA10Z335)

Research Progress in Aerobic Respiration Metabolism of Lactic Acid Bacteria

BAI Feng-ling1,2,ZHANG Bo-lin1,*,JIANG Xiang-ning1   

  1. (1. College of Biological Science and Biotechnology, Beijing Forestry University, Beijing 100083, China;
    2. College of Biotechnology and Food Science, Bohai University, Jinzhou 121000, China)
  • Received:2008-11-18 Revised:2009-01-31 Online:2009-07-01 Published:2010-12-29
  • Contact: ZHANG Bo-lin1,*, E-mail:zhangbolin888@126.com

摘要:

通过添加血红素,部分乳酸菌种(株)的细胞在有氧条件下可进行有氧呼吸代谢,细胞具有完整的呼吸链;遗传学证据表明,细胞色素氧化酶cydA 基因可以在对数生长末期获得表达,代谢调控蛋白CcpA 作为有氧代谢的“开关”进行代谢调控,此时乳酸菌细胞具有耐氧和耐酸性能力。相对于发酵条件而言,有氧代谢可促使乳酸菌细胞获得较高的生长量,培养介质的pH 值维持在较高水平,使细胞具有较强的抗逆效果和生存能力。这意味着通过控制性地改变乳酸菌的培养条件,或许能改善乳酸菌细胞在有氧条件下的增殖潜力和生存能力,拓宽其在食品发酵工业中的应用范围。

关键词: 乳酸菌, 有氧增殖, 呼吸代谢, 遗传学特征

Abstract:

Several strains (species) of lactic acid bacteria (LAB) such as Lactococcus lactis can undergo respiration metabolism and thrive in the presence of both oxygen and a heme source. Genetic evidence suggests that cydA genes encoding cytochrome d oxidase are expressed at the end of log phase, and aerobic respiration is regulated by the catabolite control protein A (CcpA) acting as“ a switch” in LAB strains, therefore the strains present some oxygen and acid resistance. Compared to fermentation, aerobic respiration can prompt effectively the achievement of high biomass, the maintenance of high pH value of culture medium and the increases of adverse resistance and survival ability. It means that the controlled changes in cultivation conditions may increase the proliferation potential and survival ability of LAB, which can widen their application scope in food fermentation industry.

Key words: lactic acid bacteria, aerobic proliferation, respiration metabolism, genetic characteristics

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