天麻中天麻素RH-HPLC测定及提取工艺研究

doi:10.7506/spkx1002-6630-200910013

食品科学 ›› 2009, Vol. 30 ›› Issue (10): 77-81.doi: 10.7506/spkx1002-6630-200910013

天麻中天麻素RH-HPLC测定及提取工艺研究

田洋¹，肖蓉^2,*，徐昆龙²，史崇颖¹，代祎²

1.云南农业大学食品科学技术学院 2.云南农业大学动物科学技术学院

收稿日期:2008-09-08 修回日期:2008-12-26 出版日期:2009-05-15 发布日期:2010-12-29
通讯作者: 肖蓉 E-mail:xiaorong91515@163.com
基金资助:
云南省自然科学基金项目(2005C0037M)

Determination of Gastrodin in Gastrodia elata Bl. by Reverse Phase (RP)-HPLC and Study on Its Extraction Technology

TIAN Yang1，XIAO Rong2,*，XU Kun-long2，SHI Chong-ying1，DAI Yi2

(1. College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China；
2. College of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China)

Received:2008-09-08 Revised:2008-12-26 Online:2009-05-15 Published:2010-12-29
Contact: XIAO Rong2,*， E-mail:xiaorong91515@163.com

摘要/Abstract

摘要：

通过考察不同检测波长、流动相和溶剂对天麻素出峰效果的影响，改进了2005 年版《中国药典》收载的天麻药材中天麻素含量测定的RP-HPLC 方法，并探讨了提取溶剂、提取温度、料液比、提取时间对天麻素提取工艺的影响。结果表明：采用Diamonsil C18 柱(150mm × 4.6mm，5μm)，乙腈- 水(3:97，V/V))溶液为流动相，流速1ml/min，柱温为室温，检测波长220nm，并用乙腈-0.05% 磷酸溶液(3:97，V/V))作为溶剂，天麻素在10～80μg/ml 浓度范围内呈现良好的线性关系，其回归方程和相关系数分别为：y ＝ 4.0264x+0.6311，R2 ＝ 0.9992，最低检测限为2ng。精密度RSD 为0.41%(n ＝ 6)，重现性实验RSD 为0.86%(n ＝ 6)，回收率为98.4%，RSD 为0.84%(n ＝ 6)；天麻素最佳提取工艺条件为：提取溶剂50% 乙醇，提取温度70℃，料液比1:10 (g/ml)，提取时间6h。

关键词: 天麻, 天麻素, RH-HPLC, 提取工艺

Abstract:

By investigating the effects of different detection wavelength, mobile phase and solvent on the peak profile of gastrodin, the present study improved the traditional RP-HPLC method for determining gastrodin in Gastrodia elata Bl.. Also, the effects of extraction solvent, extraction temperature, solid-liquid ratio and extraction time on the extraction rate of gastrodin were discussed. The results showed that the optimal working conditions of RP-HPLC were as follows: using Diamonsil C18 column (150 mm×4.6 mm, 5 μm) at ambient temperature, acetonitrile-water (3:97, V/V) as the mobile phase, flow rate 1.0 ml/min, detection wavelength 220 nm, and acetonitrile-0.05% phosphoric acid (3:97, V/V) as the solvent of gastrodin. The calibration curve was well linear in the range of 10 to 80 μg/ml, the regression equation was y = 4.0264x + 0.6311 with a determination coefficient of 0.9992. The limit of detection of this method was 2 ng. The relative standard deviations (RSDs) of precision and repeatability were 0.41% and 0.86%, respectively (n = 6), and the average spike recovery rate was 98.4% with a RSD of 0.84% (n = 6). The optimum extraction conditions of gastrodin from Gastrodia elata Bl. were determined as follows: 50% alcohol as extraction solvent, temperature 70 ℃, ratio of solid to liquid 1:6 (g/ml), and extraction time 3 h.

Key words: Gastrodia elata Bl., gastrodin, RP-HPLC, extraction technology

中图分类号:

O658

田洋1，肖蓉2,*，徐昆龙2，史崇颖1，代祎2. 天麻中天麻素RH-HPLC测定及提取工艺研究[J]. 食品科学, 2009, 30(10): 77-81.

TIAN Yang1，XIAO Rong2,*，XU Kun-long2，SHI Chong-ying1，DAI Yi2. Determination of Gastrodin in Gastrodia elata Bl. by Reverse Phase (RP)-HPLC and Study on Its Extraction Technology[J]. FOOD SCIENCE, 2009, 30(10): 77-81.

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天麻中天麻素RH-HPLC测定及提取工艺研究

Determination of Gastrodin in Gastrodia elata Bl. by Reverse Phase (RP)-HPLC and Study on Its Extraction Technology

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