食品科学 ›› 2009, Vol. 30 ›› Issue (10): 118-122.doi: 10.7506/spkx1002-6630-200910022

• 工艺技术 • 上一篇    下一篇

利用大孔吸附树脂DA201-C Ⅱ对燕麦蛋白水解液脱盐的研究

任 清1,张晓平1,赵世锋2,蒋 琼1,刘永国1,董银卯1 ,*   

  1. 1.北京工商大学 植物资源研究开发北京市重点实验室 2.张家口市农业科学院
  • 收稿日期:2008-08-07 修回日期:2008-12-01 出版日期:2009-05-15 发布日期:2010-12-29
  • 通讯作者: 董银卯1 ,* E-mail:ymdong2008@163.com

Study on Desalination of Oat Protein Hydrolysate with Macroporous Adsorption Resin DA201-CⅡ

REN Qing1,ZHANG Xiao-ping1,ZHAO Shi-feng2,JIANG Qiong1, LIU Yong-guo1, DONG Yin-mao1,*   

  1. (1. Beijing Key Laboratory of Plant Resources Research and Development, Beijing Technology and Business University, Beijing
    100037, China;2. Zhangjiakou Academy of Agricultural Sciences, Zhangjiakou 075000, China)
  • Received:2008-08-07 Revised:2008-12-01 Online:2009-05-15 Published:2010-12-29
  • Contact: DONG Yin-mao1,* E-mail:ymdong2008@163.com

摘要:

采用大孔吸附树脂DA201-C Ⅱ对燕麦蛋白水解液进行脱盐处理是行之有效的方法。静态吸附和解吸实验表明,大孔吸附树脂DA201-C Ⅱ对燕麦肽的吸附性能优于其他七种树脂,其最佳工艺条件为:pH4.0、多肽浓度39mg/ml 的酶解液以1BV/h 的流速上样、1BV/h 的流速水洗、1.5ml/min 的75% 乙醇解吸。在此条件下,大孔吸附树脂DA201-C Ⅱ对水解液的脱盐率为93.4%,燕麦肽的回收率可达72.3%。

关键词: 大孔吸附树脂, 燕麦肽, 脱盐, 吸附, 解析

Abstract:

Several macroporous adsorption resins were applied to desalination of oat protein hydrolysate. The experiments of static adsorption and desorption showed that macroporous adsorption resin DA201-CⅡhad the highest adsorption-desorption capability and to oat peptides among 8 resins. In further study, the optimal desalination conditions of oat protein hydrolysate with the resin DA201-CⅡwere confirmed as follows: pH of oat protein hydrolysate 4.0, concentration of oat peptides 39 mg/ml, flow rate of water washing 1 BV/h, and desorption rate of 75% ethanol 1.5 ml/min. Under these conditions, the desaltion rate reached about 93.4%, and the recovery of oat peptides was about 72.3%.

Key words: macroporous adsorption resin (MAR), oat peptides, desalination, adsorption, desorption

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