食品科学 ›› 2009, Vol. 30 ›› Issue (10): 156-158.doi: 10.7506/spkx1002-6630-200910031

• 分析检测 • 上一篇    下一篇

罗丹明B光谱探针测定面粉中过氧化苯甲酰

杜 娟,孙 丹   

  1. 襄樊学院化学与生物科学系
  • 收稿日期:2008-07-30 修回日期:2008-09-05 出版日期:2009-05-15 发布日期:2010-12-29
  • 通讯作者: 杜娟 E-mail:dujuan72@126.com
  • 基金资助:

    湖北省教育厅科学技术研究项目(D20082504)

Determination of Benzoyl Peroxide in Flour by Spectral Probe of Rhodamine B

DU Juan,SUN Dan   

  1. (Department of Chemistry and Biological Science, Xiangfan College, Xiangfan 441053, China)
  • Received:2008-07-30 Revised:2008-09-05 Online:2009-05-15 Published:2010-12-29
  • Contact: DU Juan, E-mail:dujuan72@126.com

摘要:

研究在磷酸氢二钠- 磷酸二氢钾缓冲溶液中(pH 6. 84),以罗丹明B 为光谱探针,分光光度法测定面粉中过氧化苯甲酰(BPO)的方法。结果表明,室温下,罗丹明B-BPO 体系在550nm 波长处有最大吸收,表观摩尔吸光系数(ε)为 1.1 × 104 L/mol·cm。BPO 含量在0.00~0.80mg/ml 范围内符合朗伯比尔定律。检出限为0.056mg/kg,加标回收率为97.5%~101.0% ,RSD 为1.46%~2.53%。该法简便易行,适用于基层单位测定面粉中微量BPO。

关键词: 罗丹明B, 过氧化苯甲酰, 光谱探针, 面粉

Abstract:

A new oxidation system, in Na2HPO4-KH2PO4 buffer (pH 6.84), with rhodamine B as the spectral probe was proposed for determination of benzoyl peroxide (BPO) in flour. Results indicated that this rhodamine B-BPO had an absorption maximum at 550 nm and the apparent molar absorption coefficient was 1.1×104 L/mol·cm. Beer's law was obeyed within the concentration of 0.00 to 0.80 mg/ml. The BPO detection limit by this method was 0.056 mg/ kg. The spike recoveries were between 97.5% and 101.0%, and the RSD were between 1.46% and 2.53%. Due to its easy operation and high recovery, this method could be applied directly to analyze trace benzoyl peroxide in flour.

Key words: rhodamine B, benzoyl peroxide, spectral probe, flour

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