食品科学 ›› 2009, Vol. 30 ›› Issue (10): 231-235.doi: 10.7506/spkx1002-6630-200910054

• 分析检测 • 上一篇    下一篇

水产品中恩诺沙星残留的一步法酶联免疫检测研究

李宗妍,曹立民* ,林 洪,隋建新   

  1. 中国海洋大学食品安全实验室
  • 收稿日期:2008-08-06 修回日期:2008-12-22 出版日期:2009-05-15 发布日期:2010-12-29
  • 通讯作者: 曹立民 E-mail:caolimin@ouc.edu.cn
  • 基金资助:

    国家自然科学基金项目(30400336); 国家农业行业研究专项(NYHYZX 07-046)

Development of One-step Enzyme-linked Immunoassay for Determining Enrofloxacin Residues in Sea Foods

LI Zong-yan,CAO Li-min*,LIN Hong,SUI Jian-xin   

  1. (Food Safety Laboratory, Ocean University of China, Qingdao 266003, China)
  • Received:2008-08-06 Revised:2008-12-22 Online:2009-05-15 Published:2010-12-29
  • Contact: CAO Li-min*, E-mail:caolimin@ouc.edu.cn

摘要:

采用过碘酸钠氧化法合成了辣根过氧化物酶(HRP)标记的恩诺沙星抗体,建立一步式直接竞争酶联免疫吸附检测(ELISA)技术,并以鳗鱼为样本进行了恩诺沙星残留的加标检测。结果表明,所制备的酶标记抗体效价达到10000 以上,与同族其他药物及族外药物没有显著的交叉反应;所建立的一步式ELISA 法检测限约为10μg/kg。在10~40μg/kg 浓度范围内,加标鳗鱼样本中恩诺沙星的检测回收率在70% 以上,相对平均偏差小于6%,检测时间缩短到2h 以内,约为传统两步式ELISA 法的一半左右。

关键词: 恩诺沙星, 酶标记抗体, 免疫检测, 水产品

Abstract:

In this study, horseradish peroxidase (HRP) was used to label anti-enrofloxacin antibody through a sodium periodate oxidation method. A one-step competitive direct enzyme-linked immunosorbent assay (ELISA) was developed for determining enrofloxacin in sea foods. The results showed that the titer of labeled antibody was was higher than 10000, and no significant cross-reactivity was observed with other fluoroquinolones and other groups of antibiotics. The detection limit was 10 μg/kg. For eel samples spiked with enrofloxacin from 10 to 40 μg/kg, the average recoveries were above 70% and the relative standard deviations were lower than 6%. The determination time by this method was shortened to less than 2 h due to pre-coating and blocking in microplates, which was about half of that by traditional two-step ELISA.

Key words: enrofloxacin, enzyme labeled antibody, immunoassay, sea food

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