食品科学 ›› 2009, Vol. 30 ›› Issue (9): 47-50.doi: 10.7506/spkx1002-6630-200909010

• 基础研究 • 上一篇    下一篇

剑麻花提取物的抗氧化活性研究

徐文汇,魏玉西*,郭 奇   

  1. 青岛大学生物系
  • 收稿日期:2008-07-16 修回日期:2008-12-08 出版日期:2009-05-01 发布日期:2010-12-29
  • 通讯作者: 魏玉西 E-mail:yuxiw729@163.com
  • 基金资助:

    岛市科技局资助项目(06-2-2-12-jch)

Study on Antioxidant Activities of Different Fractions of Ethanol Extract and Polysaccharides of Sisal Flower

XU Wen-hui WEI Yu-xi* GUO Qi   

  1. Department of Biological, Qingdao University, Qingdao 266071, China
  • Received:2008-07-16 Revised:2008-12-08 Online:2009-05-01 Published:2010-12-29
  • Contact: WEI Yu-xi E-mail:yuxiw729@163.com

摘要:

本实验分别就剑麻花乙醇提取物石油醚相、乙酸乙酯相和正丁醇相以及剑麻花多糖对DPPH·、·OH和O2·三种自由基的清除率进行了测定,并与合成抗氧化剂TBHQ、BHT进行对比。结果表明,剑麻花提取物各相对DPPH·、·OH和O2·三种自由基均有一定的清除效果。且清除率与浓度存在剂量依赖关系。其中以乙酸乙酯相对DPPH·的清除效率最高,而正丁醇相对O2·清除率最高;在浓度为64μg/ml下剑麻花提取物石油醚相对·OH的清除率最高,而乙酸乙酯相和正丁醇相对·OH的清除率与合成抗氧化剂TBHQ和BHT相近。提示剑麻花提取物石油醚相是一种潜在的羟自由基清除剂。另外,在相同浓度下,剑麻花多糖对·OH 和O2·的清除率均低于剑麻花乙醇提取物各相。

关键词: 剑麻花, 提取物, 抗氧化, 自由基清除率

Abstract:

In this study, petroleum ether-phase, ethyl acetate-phase and n-butanol-phase fractions of ethanol extract of sisal flower and polysaccharides extracted from the ethanol extraction residue were prepared and then their antioxidant activities were evaluated by measuring the scavenging rates to DPPH radical (DPPH·), hydroxyl radical (·OH) and superoxide anion radical (O2·) respectively . The results showed that the three fractions of ethanol extract all have certain activity to scavenge DPPH·, ·OH and O2· with dose-dependent manner. Furthermore, compared to the other two, the ethyl acetate-phase fraction of ethanol extract at the concentration of more than 600 μg/ml presents the highest scavenging rate to DPPH·, which is much lower than that of TBHQ and BHT. At the concentration of not less than 64μg/ml, the ethyl acetate-phase fraction exhibits the highest ·OH scavenging rate, followed by the other two fractions having similar ·OH scavenging rate to TBHQ and BHT, and the polysaccharides in turn. At the concentration of 600 μg/ml, the n-butanol-phase fraction exhibits the highest O2· scavenging rate, which is much lower than that of TBHQ and BHT, while the O2· scavenging rate of the polysaccharides is significantly lower than that of the three fractions of    ethanol extract.

Key words: sisal flower, extract, antioxidation, radical scavenging rate

中图分类号: