食品科学 ›› 2009, Vol. 30 ›› Issue (9): 73-76.doi: 10.7506/spkx1002-6630-200909017

• 基础研究 • 上一篇    下一篇

脉冲电场对大豆分离蛋白分散体系颗粒粒径的影响

刘燕燕1,曾新安1,刘克放2   

  1. 1. 华南理工大学轻工与食品学院
    2. 中山大学附属第三医院药剂科
  • 收稿日期:2008-07-23 修回日期:2008-12-29 出版日期:2009-05-01 发布日期:2010-12-29
  • 通讯作者: 刘燕燕 E-mail:liuyanyanhenan@163.com
  • 基金资助:

    广东省科技计划项目(2007B080401010)

Effect of Pulsed Electric Field on Size Distribution of Soy Protein Isolate Dispersion System

LIU Yan-yan1 ZENG Xin-an1,* LIU Ke-fang2   

  1. 1.College of Light Industry and Food Science, South China University of Technology, Guangzhou 510640, China
    2.Department of Pharmaceutical, The Third Affiliated Hospital of SUN YAT-SEN University, Guangzhou 510630, China
  • Received:2008-07-23 Revised:2008-12-29 Online:2009-05-01 Published:2010-12-29
  • Contact: LIU Yan-yan E-mail:liuyanyanhenan@163.com
  • Supported by:

    广东省科技计划项目(2007B080401010)

摘要:

本实验研究了高强脉冲电场(PEF)处理对大豆分离蛋白(SPI)多分散体系及其11 S和7 S亚基分散体系颗粒粒径分布的影响。结果表明,随着脉冲电场场强的增大,大豆分离蛋白分散体系颗粒粒径明显变小。大豆分离蛋白多分散体系及7S分散体系的多相分散指数(PDI)随着脉冲场强的增大而先增大后减小,但都大于未处理样;对于11S分散体系, PDI值却随着脉冲场强的增大而先减小后增大。同时发现20~100nm区间内的蛋白质颗粒数目增多,说明脉冲电场处理对蛋白质胶体的粒度分布有较大影响,推测可能增强体系胶体性质。

关键词: 脉冲电场(PEF), 大豆分离蛋白(SPI), 颗粒粒径分布, 多相分散指数(PDI)

Abstract:

Effects of pulsed electric field (PEF) treatment on size distribution of soy protein isolate (SPI) polydispersion system, 7S dispersion system and 11S dispersion system were investigated in this study. The results indicated that the average protein granule size significantly decreases with the increase of electric field strength. It was demonstrated that the polydispersibility index (PDI) values of SPI polydispersion system and 7S monodispersion system both increase firstly and then decrease with the increase of electric field strength, but they are higher than those of the untreated systems. On the contrary, the PDI value of 11S dispersion system decreases at first and then increases with the increase of electric field strength. Meanwhile, the quantity of protein granules in the range of 20 to 100 nm increases with the increase of electric field strength for all the tested samples, which implied that PEF treatment can greatly affect the size distribution of protein granule, thus enhance its colloid properties.

Key words: pulsed electric field (PEF), oybean protein isolate (SPI), granule size distribution, dispersibility index (DPI)

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