利用基体辅助激光解吸/电离飞行时间质谱分析水温对奶粉蛋白质的影响

doi:10.7506/spkx1002-6630-200909003

食品科学 ›› 2009, Vol. 30 ›› Issue (9): 21-24.doi: 10.7506/spkx1002-6630-200909003

利用基体辅助激光解吸/电离飞行时间质谱分析水温对奶粉蛋白质的影响

王正方¹,邹明强¹,齐小花¹,张卓勇²

1.中国检验检疫科学研究院 2.首都师范大学化学系

收稿日期:2008-08-01 修回日期:2008-12-29 出版日期:2009-05-01 发布日期:2010-12-29
通讯作者: 邹明强 E-mail:mingqiangz@sina.com
基金资助:
“十一五”国家科技支撑计划项目(2006BAK10B06;2006BAK10B09) 中国检验检疫科学研究院科研业务费资助项目(2007JK010)

Analysis on Thermal Denaturation of Proteins in Infant Formula Milk Powder Diluted with Water at Different Temperatures Using Matrix-assisted Laser Desorption Ionization Time-of-flight Mass Spectrometry (MALDI-TOF/MS )

WANG Zheng-fang1,2 ZOU Ming-Qiang1,* QI Xiao-hua1 ZHANG Zhuo-Yong2

1.Chinese Academy of Inspection and Quarantine, Beijing 100123, China
2. Department of Chemistry, Capital Normal University, Beijing 100037, China

Received:2008-08-01 Revised:2008-12-29 Online:2009-05-01 Published:2010-12-29
Contact: ZOU Ming-Qiang E-mail:mingqiangz@sina.com

摘要/Abstract

摘要：

利用基体辅助激光解吸/电离飞行时间质谱法(matrix-assisted laser desorption/ionization mass spectrometry，MALDI-TOF-MS)分析不同水温冲调下奶粉中部分蛋白质相对含量的变化，从而获得其蛋白质成分的变化规律。实验选取11个目标离子峰，对样品进行15次平行测定，每个目标离子峰每次测得的m/z值相差均不超过3，相对误差不超过0.19%，相对标准偏差不超过0.19%。最终数据为15个平行实验结果的切尾平均值。结果表明，随着水温的升高，奶粉中蛋白质逐渐发生热变性；当水温高于70℃时，奶粉蛋白变性程度开始显著增加。推荐以40℃左右水冲调奶粉为宜。

关键词: 基体辅助激光解吸/电离飞行时间质谱, 奶粉蛋白质, 热变性

Abstract:

MALDI-TOF/MS was employed to analyze the relative contents of proteins in infant formula milk powder diluted with water at different temperatures to make clear the change law of protein components of the milk powder. In this experiment, 11 ion peaks in the MALDI-TOF mass spectrum were selected as targets, and 15 parallel tests were performed on each sample. For every target ion, the m/z differed no more than 3 between any two of 15 determinations, the relative error was less than 0.19%, and the RSD was less than 0.19%. The final data were the trimmed values of all the parallel tests. It was indicated that proteins in the milk powder denature gradually with the rising of water temperature, and their denaturation occurrs at a higher degree when water temperature reached 70 ℃. In conclusion, water at 40 ℃ is proper for diluting infant formula milk powder.

Key words: MALDI-TOF/MS, proteins in infant formula milk powder, denaturation

中图分类号:

R151

王正方1,2，邹明强1,*，齐小花1，张卓勇2. 利用基体辅助激光解吸/电离飞行时间质谱分析水温对奶粉蛋白质的影响[J]. 食品科学, 2009, 30(9): 21-24.

WANG Zheng-fang1,2 ZOU Ming-Qiang1,* QI Xiao-hua1 ZHANG Zhuo-Yong2. Analysis on Thermal Denaturation of Proteins in Infant Formula Milk Powder Diluted with Water at Different Temperatures Using Matrix-assisted Laser Desorption Ionization Time-of-flight Mass Spectrometry (MALDI-TOF/MS )[J]. FOOD SCIENCE, 2009, 30(9): 21-24.

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利用基体辅助激光解吸/电离飞行时间质谱分析水温对奶粉蛋白质的影响

Analysis on Thermal Denaturation of Proteins in Infant Formula Milk Powder Diluted with Water at Different Temperatures Using Matrix-assisted Laser Desorption Ionization Time-of-flight Mass Spectrometry (MALDI-TOF/MS )

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