食品科学 ›› 2009, Vol. 30 ›› Issue (9): 33-36.doi: 10.7506/spkx1002-6630-200909006

• 基础研究 • 上一篇    下一篇

鹿茸超临界提取物体外抗氧化活性分析

周 冉,李淑芬*,张大成   

  1. 天津大学化工学院,绿色合成与转化教育部重点实验室
  • 收稿日期:2008-08-07 修回日期:2008-11-19 出版日期:2009-05-01 发布日期:2010-12-29
  • 通讯作者: 李淑芬 E-mail:shfli@tju.edu.cn
  • 基金资助:

    天津市应用基础研究计划项目(06YFJMJC10500)

Analysis on in vitro Antioxidant Activity of Supercritical Extract of Antler Velvet

ZHOU Ran LI Shu-fen* ZHANG Da-cheng   

  1. Key Laboratory for Green Chemical Technologies, Ministry of Education, School of Chemical Engineering and Technology,
    Tianjin University, Tianjin 300072, China
  • Received:2008-08-07 Revised:2008-11-19 Online:2009-05-01 Published:2010-12-29
  • Contact: LI Shu-fen E-mail:shfli@tju.edu.cn

摘要:

本实验利用以卵磷脂为模型的脂质体系、邻二氮菲-Fe2+体系产生的羟基自由基体系和脱氧核糖-铁体系产生的羟基自由基体系来研究鹿茸超临界提取物的抗氧化活性,并与VC溶液、鹿茸75%乙醇和甲醇回流提取物抗氧化活性进行比较,结果表明:三种鹿茸提取物均有一定的抗脂质和抗自由基氧化的作用,三者对邻二氮菲-Fe2+体系产生的羟基自由基的抑制作用均呈一定的量效关系;70%的乙醇提取物和甲醇提取物对脱氧核糖-铁体系产生的羟自由基的抑制作用呈一定的量效关系。超临界提取技术比传统的回流提取技术显现出明显的优势,鹿茸超临界提取物对脱氧核糖-铁体系产生的羟基自由基的抑制效果明显高于鹿茸回流提取物和VC溶液。

关键词: 鹿茸, 抗氧化, 超临界萃取

Abstract:

The antioxidant activity of supercritical extract of antler velvet in lipid peroxidation system and its hydroxyl radical-scavenging activities in phenanthroline-Fe (II) system and 2-deoxy-D-ribose-Fe (III) system were analyzed in this study, and these activities were compared to those of vitamin C, and 75% ethanol refluxing extract and methanol refluxing extract of antler velvet. The results showed that all the three antler velvet extracts have excellent activities in the above three antioxidant activity assays. Their hydroxyl radical-scavenging activities in phenanthroline-Fe (II) system all exhibit certain concentration-dependent fashion, and both 75% ethanol refluxing extract and methanol refluxing extract of antler velvet can scavenge the hydroxyl radical generated in 2-deoxy-D-ribose-Fe (III) system with a concentration-dependent manner. The supercritical extract technology shows obvious advantage, namely the supercritical extract antler velvet possesses stronger scavenging effect to the hydroxyl radical generated in 2-deoxy-D-ribose-Fe (III) system than other two antler velvet extracts and vitamin C solution do.

Key words: antler velvet, antioxidant activity, supercritical extraction

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