食品科学 ›› 2009, Vol. 30 ›› Issue (9): 152-154.doi: 10.7506/spkx1002-6630-200909036

• 生物工程 • 上一篇    下一篇

实时荧光PCR定性定量检测转基因番茄“华番一号"

邓鸿铃,覃 文   

  1. 广东食品药品职业学院
  • 收稿日期:2008-09-01 修回日期:2008-12-14 出版日期:2009-05-01 发布日期:2010-12-29
  • 通讯作者: 邓鸿铃 E-mail:zsu05@126.com
  • 基金资助:

    广东省科技厅科技专项课题(2005B80501010)

Quantitative Real-time PCR Detection of Genetically Modified Tomato Bioscein

DNEG Hong-ling QIN Wen   

  1. Guangdong Food and Drug Vocational College , Guangzhou 510520, China
  • Received:2008-09-01 Revised:2008-12-14 Online:2009-05-01 Published:2010-12-29
  • Contact: DNEG Hong-ling E-mail:zsu05@126.com

摘要:

本实验采用了特异性的引物和探针,对转基因番茄“华番一号”进行实时荧光PCR检测。相对检测灵敏度可达到0.05%。可用于转基因番茄“华番一号”的定性和定量检测。

关键词: 实时荧光PCR, 转基因番茄“华番一号”

Abstract:

Using specific primers and probes, genetically modified tomato Bioscein were detected by real-time PCR method. The relative sensitivity of this method was 0.05%. Therefore, this method can be used for qualitative and quantitative detection of Bioscein.

Key words: real-time PCR, genetically modified tomato Bioscein

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