食品科学 ›› 2009, Vol. 30 ›› Issue (9): 137-139.doi: 10.7506/spkx1002-6630-200909032

• 生物工程 • 上一篇    下一篇

微生物发酵法提取亚麻木脂素

马莹莹,钟 瑜,张春枝*   

  1. 大连工业大学生物与食品工程学院
  • 收稿日期:2008-08-31 修回日期:2008-12-24 出版日期:2009-05-01 发布日期:2010-12-29
  • 通讯作者: 马莹莹 E-mail:jinxiazhiyue@163.com
  • 基金资助:

    辽宁省教育厅资助项目(2007R03;2006T031)

Extraction Technology of Flaxseed Secoisolariciresinol Diglucoside by Microbial Fermentation

MA Ying-ying ZHONG Yu ZHANG Chun-zhi*   

  1. College of Biological and Food Engineering, Dalian Polytechnic University, Dalian 116034, China
  • Received:2008-08-31 Revised:2008-12-24 Online:2009-05-01 Published:2010-12-29
  • Contact: MA Ying-ying E-mail:jinxiazhiyue@163.com

摘要:

亚麻木脂素(SDG)是一种植物雌激素,具有多种生理功能。按2.5%的接种量将米曲霉Asp.oryzae 39#接种于亚麻籽粉,25~30℃发酵120h,再经丙酮提取,亚麻木脂素粗提物得率为21%~22%,SDG得率为16‰~17‰。发酵法提取SDG的提取率比未经发酵提高了50%。

关键词: 亚麻木脂素, 微生物发酵, 提取

Abstract:

Some studies confirmed that microbial fermentation of flaxseed meal is helpful to increase extraction yield of secoisolariciresinol diglucoside (SDG) from it. This conclusion was also proved in the present study, and Asp.oryzae 39# was screened as the fermentation strain due to its higher increasing effect on extraction yield of SDG than that of Asp. niger 848 and yeast. Furthermore, effects of fermentation conditions (such as fermentation time, temperature, and inoculation amount) of Asp.oryzae 39# on extraction yield of SDG were investigated. Results showed that the better fermentation time, temperature, and inoculation amount were 120 h, 25 to 30 ℃, and 2.5%, respectively. Under the above conditions the extraction yield of SDG was up to 21% to 22% and the SDG content in the extract is 16‰ to 17‰.

Key words: secoisolariciresinol diglucoside, microbial fermentation, extraction

中图分类号: