关键词: 古尼虫草, 提取, 分离, 抗肿瘤

Abstract:

In the present study, 30 g of Cordyceps gunni (Berk) Berk mycelia were extracted with 200 ml of methanol, and then the obtained extract was extracted the mixed 100 ml of methanol, 100 ml of deionized water and 100 ml of n-hexane and the methanol phase was collected, finally the methanol phase was extracted with 100 ml of ethyl acetate and the ethyl acetate phase was collected, concentrated through rotary evaporation, and dried for further studies, such as purification by silica thin-layer chromatography and column chromatography with diethyl ether- n-hexane-methanol (5:5:1,V/V), structural analysis of by infrared (IR) spectrometry and nuclear magnetic resonance (NMR) and anti-proliferation effects against MCF-7 and HL-7702 cells. The results showed that after the sequential solvent extraction with ethanol, n-hexane and ethyl acetate, 0.6914 g of final extract is obtained and the yield is 2.06%. The final extract comprises three fractions, namely FB1, FB2 and FB3. The fraction FB3 is proved to be a sterol compound by IR and 1H-NMR, and its has strong inhibitory effects against proliferation of MCF-7 and HL-7702 cells with the maximum inhibitory rates of 49.78% at 12.5μg/ml and 84.54% at 50.0μg/ml, respectively.

Key words: Cordyceps gunnii (Berk.) Berk, extraction, isolation, anti-tumor activity