食品科学 ›› 2009, Vol. 30 ›› Issue (9): 225-229.doi: 10.7506/spkx1002-6630-200909055

• 专题论述 • 上一篇    下一篇

微生物胆固醇氧化酶的研究进展

张 玲,杨海麟,孙 燕,霍惠芝,王 武*   

  1. 江南大学 工 业生物技术教育部重点实验室
  • 收稿日期:2008-07-24 修回日期:2008-11-25 出版日期:2009-05-01 发布日期:2010-12-29
  • 通讯作者: 王武 E-mail:wangwu@jiangnan.edu.cn
  • 基金资助:

    工业生物技术教育部重点实验室基金项目(KLIB-KF200505) 国家“863”计划项目(2006AA10Z305)

Microbial Cholesterol Oxidase: sources, physical properties and applications

ZHANG Ling YANG Hai-lin SUN Yan HUO Hui-zhi WANG Wu*   

  1. Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China
  • Received:2008-07-24 Revised:2008-11-25 Online:2009-05-01 Published:2010-12-29
  • Contact: WANG Wu E-mail:wangwu@jiangnan.edu.cn

摘要:

微生物来源的胆固醇氧化酶在医疗诊断、食品加工、生化制药、抗虫基因工程等方面具有良好的应用价值,引起了国内外学者的广泛关注,并开展了大量的研究工作。本文就胆固醇氧化酶的种类、酶学性质、结构、功能、应用及近年来基因工程等方面的研究进展作一综述。

关键词: 胆固醇氧化酶, 酶学性质, 结构, 应用, 基因工程

Abstract:

Recently, microbial cholesterol oxidase (COD) has drawn too much attention and lots of related studies have been done due to their perfect application values in clinical diagnosis, food processing, biopharmaceutical industry, and gene engineering, etc. This article reviewed the recent research progress on COD from the following aspects: (1) variety of available bacterial sources, (2) enzymatic properties, (3) molecular structure basis, (4) methodology in COD activity assay, (5) COD gene cloning, and (6) applications of COD in clinical diagnosis, food processing, biopharmaceutical industry, molecular biological study and disinsection.

Key words: cholesterol oxidase, enzymatic properties, structure, application, gene engineering

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