食品科学 ›› 2009, Vol. 30 ›› Issue (8): 47-50.doi: 10.7506/spkx1002-6630-200908006

• 工艺技术 • 上一篇    下一篇

黑芝胞外多糖提取工艺条件优化及体外抗氧化活性研究

叶 明,陈九山,杨 柳,谭 炜,孙汉巨   

  1. 合肥工业大学生物与食品工程学院
  • 收稿日期:2008-06-20 修回日期:2008-08-30 出版日期:2009-04-15 发布日期:2010-12-29
  • 通讯作者: 叶明 E-mail:forevercredit@126.com
  • 基金资助:

    安徽省自然科学基金项目(090411086);安徽省技术创新项目(2008991,2008QTXM0812)

Optimization of Extraction Technology of Extracellular Polysaccharide from Fermentation Broth Ganoderma atrum and Study on Its Antioxidant Activity in vitro

YE Ming,CHEN Jiu-shan,YANG Liu,TAN Wei,SUN Han-ju   

  1. (College of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China)
  • Received:2008-06-20 Revised:2008-08-30 Online:2009-04-15 Published:2010-12-29
  • Contact: YE Ming, E-mail:forevercredit@126.com

摘要:

本研究采用改进的苯酚-硫酸法测定黑芝多糖含量,以多糖提取率为评价指标,在单因素试验的基础上,进行正交试验,考察乙醇浓度、提取时间、发酵液pH值三个因素对多糖得率的影响。结果表明,最佳提取工艺条件为乙醇浓度80%、提取时间19h、发酵液pH5.5。在此条件下黑芝胞外多糖得率为5.2343g/L。黑芝多糖浓度为40mg/ml时,对·OH与DPPH自由基的清除率分别达到39.85%和23.4%,表明黑芝多糖具有明显的抗氧化活性。

关键词: 黑芝, 胞外多糖, 优化, 正交试验, 体外抗氧化 

Abstract:

The content of polysaccharide of Ganoderma atrum was determined by the method of improved phenol-sulphate acid. On the basis of single-factor tests, orthogonal design was applied to analyze the effects of ethanol concentration, extraction time and broth pH value on the extraction rate of polysaccharides. The results showed that the optimum ethanol concentration, extraction time and broth pH value are 80%, 19 h, and 5.5, respectively. Under these conditions, the extraction yield of extracellular polysaccharide is 5.2343 g/L. At the concentration of 40 mg/ml, the hydroxyl and DPPH radicals-scavenging activities of the polysaccharide are 39.85% and 23.4%, respectively. It indicated that the extracellular polysaccharide produced by Ganoderma atrum has a significant antioxidant activity.

Key words: Ganoderma atrum, extracellular polysaccharide, optimization, orthogonal test, antioxidant activity in vitro

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