食品科学 ›› 2009, Vol. 30 ›› Issue (8): 128-131.doi: 10.7506/spkx1002-6630-200908025

• 工艺技术 • 上一篇    下一篇

胡芦巴中4-羟基异亮氨酸的提取分离工艺研究

李玉山   

  1. 四川农业大学信息与工程技术学院
  • 收稿日期:2008-07-06 修回日期:2008-11-23 出版日期:2009-04-15 发布日期:2010-12-29
  • 通讯作者: 李玉山 E-mail:lysh@163.net

Study on Extraction and Purification of 4-Hydroxyisoleucine from Trigonella foenungraecum

LI Yu-shan   

  1. (College of Information and Engineering Technology, Sichuan Agricultural University, Yaan 625014, China)
  • Received:2008-07-06 Revised:2008-11-23 Online:2009-04-15 Published:2010-12-29
  • Contact: LI Yu-shan E-mail:lysh@163.net

摘要:

优化4-羟基异亮氨酸提取纯化工艺。以甘肃天水产胡芦巴为原料,在单因素试验基础上选取料液比、提取时间、提取温度为影响因素,进行均匀设计优化,结果显示最佳提取工艺为:10倍量水在90℃下提取2.0h,共提取2次。对提取浓缩液进行了纯化,最佳工艺为:5倍量90%乙醇沉淀、732型树脂吸附、pH9的氨水在流速为0.6ml/min下洗脱,洗脱液总用量800ml,得到的4-羟基异亮氨酸含量可达40%。该工艺较先进,可应用于工业化生产。

关键词: 胡芦巴, 4-羟基异亮氨酸, 提取, 纯化

Abstract:

the extraction conditions of 4-hydroxyisoleucine from Trigonella foenungraecum planted in Tianshui of Gansu province were optimized by uniform design based on single factor tests of several factors such as  ratio of extractant to material, extraction time, temperature and extraction times. The results showed that the optimum ratio of water to material, extraction time, temperature and extraction times are 10:1 (ml/g), 2 h, 90 ℃ and twice, respectively. After concentration, the water extract of Trigonella foenungraecum was added with 90% ethanol, and then the mixture was centrifugalized to remove Trigonella foenungraecum gum. Subsequently, the supernatant was adsorbed with 732 strong acid styrene cation exchange resin. The desorption conditions of 4-hydroxyisoleucine were investigated. The results showed that the optimal addition amount of 90% ethanol is 5 times of volume of water extract of Trigonella foenungraecum. The optimal desorption conditions are as follows: ammonia water pH 9, its total amount for desoprtion 800 ml, and its flow rate for desorption 0.6 ml/min. Under the above conditions, the content of 4-hydroxyisoleucine in final product is about 40%. After used repeatedly 5 times, the resin needs regenerating.

Key words: Trigonella foenungraecum, 4-hydroxyisoleucine, extraction, purification

中图分类号: