食品科学 ›› 2009, Vol. 30 ›› Issue (8): 218-220.doi: 10.7506/spkx1002-6630-200908048

• 分析检测 • 上一篇    下一篇

蒽酮-硫酸法测定泰山灰树花中海藻糖含量的研究

李艳玲,史仁玖,张显忠,苗 苗   

  1. 泰山医学院生物科学系
  • 收稿日期:2008-06-07 修回日期:2008-09-04 出版日期:2009-04-15 发布日期:2010-12-29
  • 通讯作者: 李艳玲 E-mail:lingl816@163.com
  • 基金资助:

    泰山医学院青年科学基金项目(2007-2008)

Determination of Trehalose Content of Grifola frondosa Mycelia by Anthrone-Sulfuric Acid Method

LI Yan-ling SHI Ren-jiu ZHANG Xian-zhong MIAO Miao   

  1. (Department of Biological Sciences, Taishan Medical College, Taian 271016, China)
  • Received:2008-06-07 Revised:2008-09-04 Online:2009-04-15 Published:2010-12-29
  • Contact: LI Yan-ling E-mail:lingl816@163.com

摘要:

对蒽酮-硫酸法测定灰树花菌体海藻糖含量的条件进行了研究。结果表明,样液与蒽酮-硫酸试剂加入量的体积比为1:2,加热显色5min,625nm比色,测定灰树花菌体中海藻糖含量为3.88mg/ml,平均回收率为106.5%,稳定性和重复性较好。该方法简便、快速、准确,适用于菌体海藻糖含量的测定。

关键词: 灰树花, 海藻糖, 蒽酮-硫酸法

Abstract:

Abstract: The procedure of colorimetric analysis of mycelium trehalose of Grifola frondosa was investigated in the present study. The results showed that when Grifola frondosa mycelium sample solution is added with anthrone-sulfuric acid reagent at half of its volume, heated at 100 ℃ for 5 min and then analyzed at 625 nm, the trehalose content in Grifola frondosa mycelium is determined as 3.88 mg/ml, and the average recovery is 106.5%. Also, the stability and repeatability of this method are good. In conclusion, this method is convenient, rapid, accurate and suitable for the determination of rehalose content in Grifola frondosa mycelium.

Key words: Grifola frondosa, trehalose, anthrone-sulfuric acid method

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