食品科学 ›› 2009, Vol. 30 ›› Issue (8): 280-283.doi: 10.7506/spkx1002-6630-200908064

• 包装贮运 • 上一篇    下一篇

奉节脐橙果皮褐变生理的研究

高雪1,李正国2   

  1. 1.韩山师范学院生物系 2.重庆大学生物工程学院
  • 收稿日期:2008-07-17 修回日期:2008-10-05 出版日期:2009-04-15 发布日期:2010-12-29
  • 通讯作者: 高雪 E-mail:gaoxue_1@163.com;snowgao1468@sina.com.cn
  • 基金资助:

    重庆市科委攻关项目(03-8001);韩山师范学院博士启动基金项目

Physiological Study on Peel Pitting of “Fengjie” Navel Orange Fruits

GAO Xue1 LI Zheng-guo2   

  1. (1. Biology Department, Hanshan Normal University, Chaozhou 521041, China
    2. College of Bio-engineering, Chongqing University, Chongqing 400044, China)
  • Received:2008-07-17 Revised:2008-10-05 Online:2009-04-15 Published:2010-12-29
  • Contact: GAO Xue1 E-mail:gaoxue_1@163.com;snowgao1468@sina.com.cn

摘要:

以奉园72-1脐橙果实为材料,进行常温(15℃)、低温(5℃)贮藏、涂蜡、机械损伤等处理,测定各处理脐橙果皮褐变率、褐变指数,以及POD、PPO、PAL酶活性的变化,以探明奉节脐橙果皮褐变的影响因素,以及相关酶活性与果皮褐变的关系。结果表明:与常温贮藏的对照果相比,涂蜡、损伤处理均极显著地提高果实的果皮褐变率(p<0.01)以及褐变指数(p<0.01),而低温贮藏可降低褐变发生;说明涂蜡和机械损伤都是引发果皮褐变的主要因素之一,低温贮藏可在一定程度上减少果皮褐变的发生。损伤处理刺激了POD、PAL酶活性的上升,且酶活性的升高与果皮褐变率具有相关性;涂蜡和损伤明显促进了PPO酶活性的升高,从而加速褐变过程,而低温可有效抑制PPO酶活性;实验结果表明POD、PPO、PAL酶活性与果皮褐变间均有密切关系。

关键词: 脐橙, 果皮褐变, PPO, POD, PAL

Abstract:

In the present study, the effects of different temperatures (15 ℃ or 5 ℃), waxing and mechanical damage on the changes in the activities of peroxide (POD), polyphenol oxidases(PPO), and phenylalanine ammonia-lyase (PAL) as well as the incidence of peel pitting in “Fengjie” navel orange (Citrus sinensis Osbeck) fruits. The results showed that the incidence of peel pitting is greatly enhanced by waxing and mechanical damage, which indicates that waxing and mechanical damage may be the important factors inducing peel pitting, while the low temperature (5 ℃) can decrease the incidence of this disease. Moreover, mechanical damage distinctly stimulates the increase of the activities of POD and PAL, and the PPO activity is promoted by both of waxing and mechanical damage, while the 5 ℃ storage treatment inhibits the PPO activity. It is suggested that the enzyme activities of POD, PPO and PAL are highly related to peel pitting occurred on “Fengjie” navel orange fruits.

Key words: navel orange (Citrus sinensis Osbeck), peel pitting, POD, PPO, PAL

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