食品科学 ›› 2009, Vol. 30 ›› Issue (7): 72-74.doi: 10.7506/spkx1002-6630-200907016

• 基础研究 • 上一篇    下一篇

山葡萄籽和皮中原花青素类SOD活性的测定

张兰杰,辛 广,王艳丽   

  1. 鞍山师范学院化学系
  • 收稿日期:2008-06-30 修回日期:2008-12-29 出版日期:2009-04-01 发布日期:2010-12-29
  • 通讯作者: 张兰杰 E-mail:zhanglanjieas@163.com
  • 基金资助:

    鞍山市科委科学研究项目(2007SF26)

Determination of Superoxide Dismutase (SOD) -like Activity of Proanthocyanidins in Seeds and Peels of  Vitaceae amurensis

ZHANG Lan-jie XIN Guang WANG Yan-li   

  1. (Department of Chemistry, Anshan Normal University, Anshan 114007, China)
  • Received:2008-06-30 Revised:2008-12-29 Online:2009-04-01 Published:2010-12-29
  • Contact: ZHANG Lan-jie E-mail:zhanglanjieas@163.com

摘要:

采用邻苯三酚自氧化法测定山葡萄籽和皮中原花青素SOD-L的活性为192.85U/g和78.45U/g。通过实验确定了适宜的测定条件:测定波长325nm,缓冲体系为 Tris-HAc缓冲液,浓度为50mmol,pH8.2。原花色素SOD-L对连苯三酚自氧化的抑制率达到91%。

关键词: 山葡萄籽和皮, 原花青素, SOD-L活性, 连苯三酚自氧化法

Abstract:

Pyrogallol autooxidation method was applied to determine SOD-like activities of proanthocyanidins in seeds and peels of Vitaceae amurensis. The results indicated that the SOD-like activities of proanthocyanidins in seeds and peels of Vitaceae amurensis are 192.85 U/g and 78.45 U/g, respectively. The optimum parameters for this assay are as follows: determining absorbance at the wavelength of 325 nm, and taking Tris-HA at pH 8.2 and at the concentration of 50 mmol/L as buffer system. The inhibition percentage of proanthocyanidins against pyrogallol antooxidation reaches 91%.

Key words: Vitaceae amurensis, proanthocyanidins, superoxide dismutase activity, pyrogallol autooxidation

中图分类号: