食品科学 ›› 2009, Vol. 30 ›› Issue (7): 159-161.doi: 10.7506/spkx1002-6630-200907036

• 生物工程 • 上一篇    下一篇

发酵液中纳他霉素的生物检测法

乔春明1,刘 坤2,葛菁萍3,胡海洋4,平文祥3   

  1. 1.哈尔滨市职工医学院 2.黑龙江辰能生物工程有限公司 3. 黑龙江省高校微生物重点实验室,黑龙江大学生命科学学院 4.哈尔滨制药总厂
  • 收稿日期:2007-12-05 修回日期:2008-11-24 出版日期:2009-04-01 发布日期:2010-12-29
  • 通讯作者: 乔春明 E-mail:qiaochunming123456@163.com

Bioassay Method of Natamycin in Fermentation Broth

QIAO Chun-ming1 LIU Kun2 GE Jing-ping3 HU Hai-yang4 PING Wen-xiang3   

  1. (1.College of Harbin Staff Medica, Harbin 100052, China 2.Heilongjiang Chenneng Bio-engineering Co., Ltd, Harbin 150090,
    China 3.Key Laboratory of Microbiology , College of Life Science, Heilongjiang University, Harbin 150080, China
    4.Harbin Pharmaceutical Group Co., Ltd. General Pharm. Factory, Harbin 150080, China)
  • Received:2007-12-05 Revised:2008-11-24 Online:2009-04-01 Published:2010-12-29
  • Contact: QIAO Chun-ming1 E-mail:qiaochunming123456@163.com

摘要:

实验对管碟法测定纳他霉素的各项参数进行了研究并量化控制。首先从16种酵母菌中筛选出最利于检测的一种酵母菌,并确定了酵母菌浓度。纳他霉素含量与抑菌圈直径平方呈良好的线性关系,回归方程为y=0.0013x-1.3369(R2=0.9965)。利用此回归方程对纳他霉素进行检测,最小检测浓度为0.1g/L,最大检测浓度为1.5g/L。

关键词: 生物检测法, 纳塔尔链霉菌, 纳他霉素, 纳他霉素发酵液

Abstract:

The parameters of agar diffusion method for determining natamycin was investigated and controlled in quantification. The results showed that there is a good linear relationship between content of natamycin and diameter square of inhibition zone, and the regression equation is y = 0.0013x-1.3369 (R2 = 0.9965). The detection range of this method is 0.1 g/L to1.5 g/L.

Key words: bioassay method, Streptomyces natalensis, natamycin, natamycin fermentation broth

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