关键词: SAS, 响应面, 茶薪菇, 纤维素酶

Abstract:

Response surface analysis was applied to optimize the fermentation conditions for cellulase production by Agrocybe chaxingu. Firstly, two-level factorial design of Plackett-Burman was used to evaluate the effect of seven related factors on produced cellulase activity, and three main factors were screened out, including carbon source, pH value and fermentation time. Then the path of steepest ascent was taken to approach the optimal region of fermentation conditions. Finally, the optimum levels of three main factors were obtained through Box-Behnken design, regression analysis with statistical analysis system and response surface analysis. Through the optimization of culture conditions, the cellulase activity is promoted by 45.26%.

Key words: statistical analysis system (SAS), response surface, Agrocybe chaxingu, cellulase