食品科学 ›› 2009, Vol. 30 ›› Issue (7): 141-144.doi: 10.7506/spkx1002-6630-200907032

• 生物工程 • 上一篇    下一篇

两歧双歧杆菌微胶囊化及其性质研究

李来酉,赵 敏*,张 帆,鹿晓菲   

  1. 东北林业大学生命科学学院
  • 收稿日期:2008-06-13 修回日期:2008-11-28 出版日期:2009-04-01 发布日期:2010-12-29
  • 通讯作者: 赵敏 E-mail:emarsn@hotmail.com
  • 基金资助:

    哈尔滨市攻关项目(0114211066);大庆市产业化资助项目

Preparation and Characterization of Bifidobacterium bifidum Microcapsule

LI Lai-you ZHAO Min* ZHANG Fan LU Xiao-fei   

  1. (College of Life Science, Northeast Forestry University, Harbin 150040, China)
  • Received:2008-06-13 Revised:2008-11-28 Online:2009-04-01 Published:2010-12-29
  • Contact: ZHAO Min* E-mail:emarsn@hotmail.com

摘要:

以明胶、果胶、海藻酸钠、氯化钙和壳聚糖为壁材,采用乳化法双层包埋制备双歧杆菌微胶囊。制备的微胶囊粒径在10~30μm。检测结果表明,微胶囊内活菌数可达到109 CFU/g以上,菌体包埋率可达到82.24%。经模拟胃酸、胆汁酸处理后活菌数仍在108 CFU/g以上,对酸有很高的耐受力;经人工肠液处理15min,微胶囊几乎全部崩解,肠溶释放率可达到95.81%。通过经典的加速实验证明微胶囊的活菌贮藏稳定性较好,室温下贮藏1年其活菌数仍可以保持在108CFU/g以上。

关键词: 双歧杆菌, 乳化法, 微胶囊, 活菌数

Abstract:

With gelatin, pectin, alginate sodium, calcium chloride and chitosan as wall materials, Bifidobacterium bifidum microcapsules were prepared by emulsification and double-layer embedment method. The determination results showed the diameter of microcapsule particles is 10 to 30  μm, the viable cell count is more than 109 CFU/g, and the embedding rate reaches 82.24%. After stimulated gastric solution or bile acid treatment, the viable cell count of Bifidobacterium bifidum in microcapsule is more than 108 CFU/g, which indicates its high acid tolerance. After the treatment of stimulated intestinal solution for 15 min, almost all microcapsules disintegrate and the release rate reaches 95.81%. The classic accelerating test proved that the Bifidobacterium bifidum microcapsules have good stability for storage. After one-year storage at room temperature, the viable cell count is still over 108 CFU/g.

Key words: Bifidobacterium bifidum, emulsification method, microcapsule, viable cell count

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