食品科学 ›› 2009, Vol. 30 ›› Issue (7): 154-158.doi: 10.7506/spkx1002-6630-200907035

• 生物工程 • 上一篇    下一篇

响应面优化褐黄孢链霉菌原生质体再生培养基

骆健美,李建姝,郭 浩,刘 峰,刘 丹,王 敏   

  1. 工业微生物教育部重点实验室,天津科技大学生物工程学院
  • 收稿日期:2008-07-10 修回日期:2008-11-13 出版日期:2009-04-01 发布日期:2010-12-29
  • 通讯作者: 骆健美 E-mail:luojianmei@tust.edu.cn
  • 基金资助:

    天津市应用基础研究计划面上项目(07JCYBJC07800); IFS国际科学基金项目 (F/4239-1)
    天津科技大学引进人才科研启动基金项目(20060426)

Optimization of Protoplast Regeneration Medium Composition for Streptomyces gilvosporeus by Response Surface Methodology

LUO Jian-mei LI Jian-shu GUO Hao LIU Feng LIU Dan WANG Min   

  1. (Key Laboratory of Industrial Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science and
    Technology, Tianjin 300457, China)
  • Received:2008-07-10 Revised:2008-11-13 Online:2009-04-01 Published:2010-12-29
  • Contact: LUO Jian-mei E-mail:luojianmei@tust.edu.cn

摘要:

本实验以再生率作为考察指标,采用单因素和响应面法优化了褐黄孢链霉菌原生质体再生培养基中的三种成分,确定其最佳浓度组合为:磷酸二氢钾0.13g/L、L-脯氨酸5.48g/L、聚乙烯吡咯烷酮0.18g/L。在优化后的再生培养基中原生质体再生率达到17.2%,与模型预测值16.7%拟合较好。结果表明,响应面法是优化原生质体再生培养基的一种有效方法。

关键词: 褐黄孢链霉菌, 原生质体, 再生培养基, 再生率, 响应面法

Abstract:

In order to promote the regeneration rate, three components of protoplast regeneration medium for Streptomyces gilvosporeus were optimized by means of single-factor design and response surface methodology. The results showed that the optimal concentrations of KH2PO4, L-proline and polyvinylpyrrolidone are 0.13, 5.48 and 0.18 g/L, respectively. Under the above conditions, the protoplast regeneration rate reaches 17.2%, which well accords with the model prediction value of 16.7%. It was indicated that response surface methodology is an effective way to optimize regeneration medium composition of protoplast.

Key words: Streptomyces gilvosporeus, protoplast, regeneration medium, regeneration rate, response surface methodology

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