关键词: 乳酸乳球菌, 胞外多糖, 纯化, 抗氧化性

Abstract:

In this study, the process conditions were investigated for purifying extracellular polysaccharide from pretreated fermentation broth (extracellular polysaccharide solution) of Lactococcus lactis subsp. lactis with macroporous resin, and the extracellular polysaccharide was administered to ICR mice by gavage to investigate its antioxidant activity. The results showed that under the conditions of adsorbing the extracellular polysaccharide solution at pH 3.5 with resin DA201-C of 2% for 20 h at 45 ℃ in static state, the reservation ratio, protein removal ratio and de-colorization ratio are 81.56%, 72.09% and 81.69%, respectively. Moreover, the animal experiment indicated that compared with the control, the antioxidant functions of serum and liver in extracellular polysaccharide-administered mice are significantly improved through enhancing CAT and SOD activities and reducing MDA contents in mouse serum and liver tissue.

Key words: Lactococcus lactis subsp. lactis, extracellular polysaccharide, purification, antioxidant ability