食品科学 ›› 2009, Vol. 30 ›› Issue (7): 225-228.doi: 10.7506/spkx1002-6630-200907051

• 营养卫生 • 上一篇    下一篇

槟榔提取物对小白鼠体内抗氧化作用的研究

袁列江1,2,李忠海1,郑锦星1   

  1. 1.中南林业科技大学食品科学与工程学院 2.国家农副产品质量监督检验中心(湖南)
  • 收稿日期:2008-06-07 修回日期:2008-08-30 出版日期:2009-04-01 发布日期:2010-12-29
  • 通讯作者: 袁列江 E-mail:yuanliejiang@163.com

in vitro and in vivo Antioxidant Activities of Varous Areca Nut Extracts

YUAN Lie-jiang1,2 LI Zhong-hai1 ZHENG Jin-xing1   

  1. (1.College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China
    2.China Agricultural Product and By-product Quality Inspection Center (Hunan), Changsha 410007, China)
  • Received:2008-06-07 Revised:2008-08-30 Online:2009-04-01 Published:2010-12-29
  • Contact: YUAN Lie-jiang1,2 E-mail:yuanliejiang@163.com

摘要:

以海南产槟榔鲜果为原料,制备槟榔提取物,研究槟榔提取物对小白鼠体内的抗氧化作用,通过体外实验筛选出来活性较好的提取物,将其作为受试物,以血清SOD及血清MDA水平为评价指标,进行小白鼠体内抗氧化作用研究。结果表明,槟榔粗提取物、乙酸乙酯萃取物和水溶出物三种组分对羟自由基(·OH)具有良好的清除作用,清除率均在53%以上。三种受试物的各个剂量均能显著提高小白鼠的血清SOD水平(p<0.05);而除乙酸乙酯萃取物高剂量作用效果不显著外(p>0.05),其他各受试物的各个剂量均能显著降低实验动物的血清MDA浓度(p<0.05)。槟榔提取物在小白鼠体内具有良好的抗氧化活性作用。

关键词: 槟榔提取物, 羟基自由基, 抗氧化作用, 超氧化物歧化酶, 丙二醛

Abstract:

The in vitro and in vivo antioxidant activities of Hainnan areca nut extract with 70% methanol aqueous solution (crude extract) and petroleum ether soluble fraction (fraction I ), chloroform soluble fraction (fraction II), ethyl acetate soluble fraction (fraction III) and water soluble fraction (fraction IV) of this extract and were investigated by measuring hydroxyl radical scavenging capacity and white mouse experiment. The results showed that the crude extract, fraction III and fraction IV present higher hydroxyl radical scavenging activity and the scavenging rates are all above 53%. In in vivo study, these three extracts are able to increase serum SOD levels at the doses of 1.0 mg/ml (low dose), 3.0 mg/ml (medium dose) and 5.0 mg/ml (high dose) by 0.1 ml/10 g bw (p<0.05), and except for the high dose of fraction III, decreases in the serum MDA content are found in mice administrated with these three extracts. In conclusion, these three extracts of areca nut have strong antioxidant activity both in vitro and in vivo.

Key words: areca nut extract, hydroxyl radical, antioxidant activity, SOD, MDA

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