食品科学 ›› 2009, Vol. 30 ›› Issue (6): 49-51.doi: 10.7506/spkx1002-6630-200906006

• 工艺技术 • 上一篇    下一篇

大叶冬青叶中总黄酮测定方法和提取工艺研究

旷春桃,李湘洲,汪玉霞,刘 慎,吴 斌   

  1. 中南林业科技大学材料科学与工程学院
  • 收稿日期:2008-04-13 修回日期:2008-06-25 出版日期:2009-03-15 发布日期:2010-12-29
  • 通讯作者: 旷春桃 E-mail:hnkct@163.com
  • 基金资助:

    中南林业科技大学青年科学研究基金资助项目(06014B)

Study on Determination Methods and Extraction Process of Total Flavonoids in Leaves of Ilex latifolia

KUANG Chun-tao,LI Xiang-zhou,WANG Yu-xia,LIU Shen,WU Bin   

  1. (College of Material Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China)
  • Received:2008-04-13 Revised:2008-06-25 Online:2009-03-15 Published:2010-12-29
  • Contact: KUANG Chun-tao, E-mail:hnkct@163.com

摘要:

采用氯化铝法、硝酸铝- 亚硝酸钠法分别测定大叶冬青叶中的总黄酮含量。实验结果表明两种方法的测定结果相差很大,通过显色机理的分析,确立了氯化铝法为大叶冬青叶总黄酮含量测定的适宜方法,方法的回收率90.7%~109.5%,平均回收率为97.8%,相对标准偏差2.2%。通过正交试验研究了大叶冬青叶中总黄酮提取工艺,结果表明其适宜工艺条件为液料比12:1(V/m)、乙醇浓度60%、提取时间2h、提取次数2 次,此条件下总黄酮得率为0.68%。

关键词: 大叶冬青叶, 总黄酮, 测定方法, 提取工艺

Abstract:

The content of total flavonoids in leaves of Ilex latifolia Thunb. were determined using aluminum chloride method and aluminum nitrate-sodium nitrite method. The experimental results showed that the determination results of content of total flavonoids by the two methods are different from each other. The analysis on mechanism of color development of two methods indicated that the aluminum chloride method is suitable for the determination of total flavonoids in leaves of Ilex latifolia Thunb.. The relative standard deviation of precision is 2.2%. The spike recovery rates are from 90.7% to 109.5% and their average value is 97.8%. The extraction process of total flavonoids from leaves of Ilex latifolia Thunb. was optimized by means of orthogonal test design. The results showed that the extraction rate of total flavonoids is 0.68% under the optimal conditions of ratio of extractant to raw material 12:1, taking 60% ethanol as extractant, extraction time 2 h and extraction times 2.

Key words: leaves of Ilex latifolia Thunb., total flavonoids, determination method, extraction process

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