食品科学 ›› 2009, Vol. 30 ›› Issue (6): 109-112.doi: 10.7506/spkx1002-6630-200906021

• 工艺技术 • 上一篇    下一篇

繁缕叶蛋白提取工艺研究

周建建,单 宇,郑玉红,管福琴,夏 冰*   

  1. 江苏省中国科学院植物研究所,江苏省药用植物研究开发中心
  • 收稿日期:2008-05-31 修回日期:2008-11-21 出版日期:2009-03-15 发布日期:2010-12-29
  • 通讯作者: 夏冰 E-mail:xiabingnbg@sina.com
  • 基金资助:

    江苏省药用植物研究开发中心2007 年开放基金项目(200701);江苏省社会公益研究和服务专项(2006507)

Study on Extraction Process of Leaf Protein Concentrate (LPC) from Stellaria media

ZHOU Jian-jian,SHAN Yu,ZHENG Yu-hong,GUAN Fu-qin,XIA Bing*   

  1. (Jiangsu Provincial Key Laborary for Medicinal Plant, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing
    210014, China)
  • Received:2008-05-31 Revised:2008-11-21 Online:2009-03-15 Published:2010-12-29
  • Contact: XIA Bing* E-mail:xiabingnbg@sina.com

摘要:

以鲜繁缕为材料,采用酸化和加热相结合的方法,研究了提取繁缕叶蛋白的最佳工艺条件。在考虑料液比、滤布层数、加热温度和加热时间等单因素对叶蛋白提取率影响的基础上,进行了正交试验,最终确定叶蛋白最佳提取工艺条件为:料液比1:5、5 层滤布过滤、加热到75℃并在此温度下持续11min,繁缕叶蛋白的提取率为52.08%,得率为27.17%。

关键词: 叶蛋白, 繁缕, 提取工艺

Abstract:

The acid-heating extraction conditions of LPC from fresh leaves of Stellaria media were optimized in this study. The effects of solid-liquid ratio, layer number of filter cloth, temperature and time on extraction rate of LPC were investigated. Based on the results of single factor tests, orthogonal test was designed to optimize the extraction conditions of LPC. The results showed that the optimal extraction conditions are as follows: solid-liquid ratio 1:5, layer number of filter cloth 5 and heating temperature 75 ℃ and heating time 11 min. Under these conditions, the extraction rate of LPC reaches 52.08% and the yield rate is 27.17%.

Key words: leaf protein concentrate (LPC), Stellaria media, extraction process

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