食品科学 ›› 2009, Vol. 30 ›› Issue (6): 72-75.doi: 10.7506/spkx1002-6630-200906012

• 工艺技术 • 上一篇    下一篇

金花茶叶中总黄酮提取及动态变化研究

黄永林,文永新* ,陈月圆,唐文秀,李典鹏,刘金磊,韦 霄   

  1. 广西植物研究所
  • 收稿日期:2008-05-27 修回日期:2008-09-02 出版日期:2009-03-15 发布日期:2010-12-29
  • 通讯作者: 文永新* E-mail:wyx@gxib.cn
  • 基金资助:

    广西壮族自治区基金项目(桂科基0639047);国家基金面上项目(30560015);
    广西壮族自治区科技攻关项目(桂科攻0718002-3-7)

Study on the Extraction Technology and Dynamic Change of Flavonoids in Camellia nitidssima Leaves

HUANG Yong-lin,WEN Yong-xin*,CHEN Yue-yuan,TANG Wen-xiu,LI Dian-peng,LIU Jin-lei,WEI Xiao   

  1. (Guangxi Institute of Botany, Guilin 541006, China)
  • Received:2008-05-27 Revised:2008-09-02 Online:2009-03-15 Published:2010-12-29
  • Contact: WEN Yong-xin*, E-mail:wyx@gxib.cn

摘要:

采用单因素及正交试验设计对金花茶叶中总黄酮提取工艺进行了研究,并以芦丁为对照品,采用分光光度法对金花茶中总黄酮动态变化规律进行了研究。结果表明:金花茶叶中总黄酮最佳提取条件为乙醇体积分数70%、料液比1:50(g/ml)、提取时间2.0h、温度70℃。1~10 月份金花茶叶中总黄酮含量呈波动性上升趋势,10月份达到最高。

关键词: 金花茶, 总黄酮, 提取工艺, 动态变化

Abstract:

Based on the results of single factor, orthogonal test design was adopted to optimize the extraction technology of total flavonoids in Camellia nitidssima leaves. With rutin as reference material, the dynamic change law of content of total flavonoids in Camellia nitidssima leave was analyzed using spectrophotometry. The results showed that the optimal extraction conditions are: using 70% ethanol as extractant, ratio of material to solution 1:50 (W/V), extraction time 2.0 h, extraction temperature 70 ℃. The content of total flavonoids in Camellia nitidssima increases fluctuatingly from January to October, reaching the highest at October.

Key words: Camellia nitidssima, total flavonoids, extraction technology, dynamic change

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