食品科学 ›› 2009, Vol. 30 ›› Issue (6): 151-154.doi: 10.7506/spkx1002-6630-200906032

• 分析检测 • 上一篇    下一篇

不同采收期广西一点红总生物碱含量分析

周吴萍,李军生,韦媛媛,阎柳娟,梁 燕   

  1. 广西工学院生物与化学工程系
  • 收稿日期:2008-03-13 修回日期:2008-06-16 出版日期:2009-03-15 发布日期:2010-12-29
  • 通讯作者: 周吴萍 E-mail:kxt_w@126.com
  • 基金资助:

    广西自然科学基金项目(桂科基0575023)

Determination of Total Alkaloids in Emilia sonchifolia Collected at Different Harvesting Time in Guangxi

ZHOU Wu-ping,LI Jun-sheng,WEI Yuan-yuan,YAN Liu-juan,LIANG Yan   

  1. (Department of Biological and Chemical Engineering, Guangxi University of Technology, Liuzhou 545006, China)
  • Received:2008-03-13 Revised:2008-06-16 Online:2009-03-15 Published:2010-12-29
  • Contact: ZHOU Wu-ping E-mail:kxt_w@126.com

摘要:

目的:建立一点红总生物碱的含量测定方法,测定不同采收期一点红中总生物碱含量,考察一点红原药材质量的优劣。方法:利用单因素法考察最佳提取条件,采用紫外可见分光光度法,以苦参碱为对照品,于414nm 波长处测定生物碱含量。结果:苦参碱在0.0021~0.0294mg/ml 范围内呈良好线性关系(R2=0.9997),平均加样回收率为97.6%,RSD 为0.447%(n=6)。样品的总生物碱含量在4.31~13.99mg/g 之间,八月为最佳采收期,其样品中总生物碱含量最高。结论:本法测定,重复性较好,对仪器要求不高,准确可靠,可用于一点红质量控制。

关键词: 一点红, 总生物碱, 苦参碱, 含量测定

Abstract:

Objective: To establish a method for determing total alkaloids content in Emilia sonchifolia and then determine its best harvesting time. Methods: The optimum extraction conditions were confirmed by single factor tests, and with matrine as standard, total alkaloids content was determined at the wavelength of 414 nm by UV-visible spectrophotometry. Results: The calibration curve shows good linearity in the range of 0.0021 to 0.0294 mg/ml (R2=0.9997), and the average spike recovery is 97.6% with the RSD of 0.447% (n=6). The contents of total alkaloids in chloroform extract samples of Emilia sonchifolia collected at different harvesting time are 4.31 to 13.99 mg/g, and August is considered as the best harvesting time of Emilia sonchifolia with the highest otal alkaloids content. Conclusion: The method is simple and accurate with high repeatability, and can be used for quality analysis of Emilia sonchifolia.

Key words: Emilia sonchifolia, total alkaloids, matrine, content determination

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