食品科学 ›› 2009, Vol. 30 ›› Issue (6): 229-231.doi: 10.7506/spkx1002-6630-200906053

• 分析检测 • 上一篇    下一篇

高效液相色谱法测定蓝莓果浆中糖的组成和含量

张英春,董爱军,杨 鑫* ,张 华,马 莺   

  1. 哈尔滨工业大学食品科学与工程学院
  • 收稿日期:2008-04-21 修回日期:2008-07-28 出版日期:2009-03-15 发布日期:2010-12-29
  • 通讯作者: 杨 鑫* E-mail:zyc229@163.com

Determination of Saccharide Components and Contents in Blueberry Juice by HPLC

ZHANG Ying-chun,DONG Ai-jun,YANG Xin*,ZHANG Hua,MA Ying   

  1. (College of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, China)
  • Received:2008-04-21 Revised:2008-07-28 Online:2009-03-15 Published:2010-12-29
  • Contact: YANG Xin*, E-mail:zyc229@163.com

摘要:

目的:建立高效液相色谱法测定蓝莓果浆中糖的组成和含量的检测方法。方法:超声波水提样品中的糖类,色谱柱为Agilent Zorbax carbohydrate 柱(4.6 × 250mm,5μm),流动相为乙腈- 水(75:25,V:V),流速为1ml/min。结果:糖类浓度在0.5~10mg/ml 内呈现良好的线性关系,相关系数在0.9989(n=5)以上;精密度实验RSD为0.36%~2.18%(n=5);平均回收率为91.36%~101.34%。结论:该方法简便快速,结果准确可靠,为测定野生蓝莓果浆中糖组分提供了一种高效快速的测定方法。

关键词: 蓝莓果浆, 果糖, 葡萄糖, 蔗糖, 高效液相色谱法

Abstract:

Objective: To set up an examination method for determining saccharides in blueberry juice by HPLC. Method: Saccharides were extracted from samples with water using ultrasonic, and then their components and contents were determined by HPLC. Agilent Zorbax carbohydrate (4.6 × 250 mm, 5 μm) column was adopted, mobile phase was acetonitrile-water (75:25, V:V), and the flow rate was 1 ml/min. Results: The calibration curve of saccharide concentrations is well linear in the range of 0.5 to 10 mg/ml, the correlation coefficient is over 0.9989 (n=5), the RSD is 0.36% to 2.18% (n=5) in precision experiment, and the average recovery rate is 91.36% to 101.34%. Conclusion: This method is convenient, rapid and effective for separation and quantitative analysis of saccharides in blueberry juice.

Key words: blueberry juice, fructose, glucose, sucrose, HPLC

中图分类号: